Detection of synthetic RGDS(PO3H2)PA peptide adsorption using a titanium surface plasmon resonance biosensor

The purpose of this study was to measure the time-dependent chemical interaction between synthetic RGDS(PO 3 H 2 )PA (P-RGD) peptide and titanium surfaces using a titanium surface plasmon resonance (SPR) biosensor and to determine the degree of peptide immobilization on the surfaces. An SPR instrume...

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Veröffentlicht in:	Journal of materials science. Materials in medicine 2011-03, Vol.22 (3), p.657-661
Hauptverfasser:	Abe, Yasuhiko, Hiasa, Kyou, Hirata, Isao, Okazaki, Yohei, Nogami, Keisuke, Mizumachi, Wataru, Yoshida, Yasuhiro, Suzuki, Kazuomi, Okazaki, Masayuki, Akagawa, Yasumasa
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Biocompatible Materials - chemistry Biological and medical sciences Biomaterials Biomedical engineering Biomedical Engineering and Bioengineering Biomedical materials Biosensing Techniques Biosensors Ceramics Chemical synthesis Chemistry and Materials Science Composites Glass Helium Hydrogen-Ion Concentration Materials Science Medical sciences Natural Materials Neon Oligopeptides - chemistry Organophosphonates - chemistry Oxygen - chemistry Peptides Peptides - chemistry Phosphates - chemistry Polymer Sciences Regenerative Medicine/Tissue Engineering Surface Plasmon Resonance Surface Properties Surfaces and Interfaces Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Thin Films Time Factors Titanium Titanium - chemistry
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Zusammenfassung:	The purpose of this study was to measure the time-dependent chemical interaction between synthetic RGDS(PO 3 H 2 )PA (P-RGD) peptide and titanium surfaces using a titanium surface plasmon resonance (SPR) biosensor and to determine the degree of peptide immobilization on the surfaces. An SPR instrument for ‘single-spot’ analysis was used for nanometer-scale detection of biomolecular adsorption using a He–Ne laser light according to Knoll’s method. The oxidized titanium surface was etched when exposed to H 3 PO 4 solutions with a pH of 2.0 or below. The amount of P-RGD adsorbed at pH 1.9 was approximately 3.6 times as much as that at pH 3.0 ( P
ISSN:	0957-4530 1573-4838
DOI:	10.1007/s10856-010-4222-2