Methyl jasmonate- and salicylic acid-induced d-chiro-inositol production in suspension cultures of buckwheat (Fagopyrum esculentum)

The aim of this work is to investigate the effects of methyl jasmonate (MeJ) and salicylic acid (SA) on d -chiro-inositol (DCI) production in buckwheat ( Fagopyrum esculentum ) suspension cultures. In this study, adding optimal concentrations of MeJ and SA at an appropriate time markedly increased DCI production (yield 6.141 and 5.521 mg/g DW, respectively). In addition, treatment of buckwheat cultures with a combination of 0.2 mM MeJ and 0.6 mM SA on days 0 and 9 increased the DCI yield to 7.579 mg/g DW, which was 3.726 times higher than that in the control; furthermore, the former yield was higher than that achieved by the addition of either elicitor alone. Moreover, unlike MeJ, SA did not exert a negative effect on cell growth.