Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads
The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads s...
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| Veröffentlicht in: | Analytical Sciences 2011/02/10, Vol.27(2), pp.213-213 |
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| container_title | Analytical Sciences |
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| creator | SAKATA, Masayo YOSHIMURA, Kana SAKAMOTO, Itsumi TODOKORO, Masami KUNITAKE, Masashi |
| description | The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 μg mL−1, pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL−1), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and |
| doi_str_mv | 10.2116/analsci.27.213 |
| format | Article |
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The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 μg mL−1, pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL−1), without the adsorption of DNA. 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SCI</addtitle><addtitle>Anal Sci</addtitle><description>The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 μg mL−1, pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL−1), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and <1%, respectively.</description><subject>Adsorption</subject><subject>Analytical Chemistry</subject><subject>beta-Cyclodextrins - chemistry</subject><subject>Buffers</subject><subject>Chemistry</subject><subject>DNA - chemistry</subject><subject>gamma-Cyclodextrins - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lipopolysaccharides - chemistry</subject><subject>Lipopolysaccharides - isolation & purification</subject><subject>Microspheres</subject><subject>Osmolar Concentration</subject><subject>Solutions</subject><issn>0910-6340</issn><issn>1348-2246</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvSK0dkiQOnbP0Rx_axpC0gVSBRerYcZ1KyOHGxk6r773G02xUgIXwYazTPvDN6B6HXlKwZpdWZHa1Prl8zmXP-DK0oL1XBWFk9RyuiKSkqXpIjdJzShhDKFGMv0VFGGS1LtUK3N-DBTf0D4K8whAfrcejw5diGKTz2I-5iGLDFF5_P8U3w89SHETdbXMeQUuH78Qe0uN46H1p4nGJueA-2Ta_Qiy7vBaf7_wTdXl1-qz8W118-fKrPrwtXlXIqdAddyWVjW2uhkqKhXAFVVGlJGZSENCBcJzURXX4aKi6hkZrpvLtoWsVP0Lud7n0MP2dIkxn65MB7O0KYk9GE8UoJQf5LKkF1dk5UmXz7F7kJc1xsNotlQmqulsnrHeUWJyJ05j72g41bQ4lZLmP2lzFM5pznhjd72bkZoD3gT6fIwNkOSLk03kH8be6_JOtdxyZN9g4OkjZOvfPwB74PlB-q7ruNBkb-C6UEslE</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>SAKATA, Masayo</creator><creator>YOSHIMURA, Kana</creator><creator>SAKAMOTO, Itsumi</creator><creator>TODOKORO, Masami</creator><creator>KUNITAKE, Masashi</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><scope>7T7</scope><scope>7TM</scope><scope>C1K</scope></search><sort><creationdate>2011</creationdate><title>Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads</title><author>SAKATA, Masayo ; 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| source | MEDLINE; Open Access Titles of Japan; SpringerLink (Online service); Free Full-Text Journals in Chemistry; EZB Electronic Journals Library; J-STAGE |
| subjects | Adsorption Analytical Chemistry beta-Cyclodextrins - chemistry Buffers Chemistry DNA - chemistry gamma-Cyclodextrins - chemistry Hydrogen-Ion Concentration Lipopolysaccharides - chemistry Lipopolysaccharides - isolation & purification Microspheres Osmolar Concentration Solutions |
| title | Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads |
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