Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads

Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads

The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads s...

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Veröffentlicht in:Analytical Sciences 2011/02/10, Vol.27(2), pp.213-213
Hauptverfasser: SAKATA, Masayo, YOSHIMURA, Kana, SAKAMOTO, Itsumi, TODOKORO, Masami, KUNITAKE, Masashi
Format: Artikel
Sprache:eng
Schlagworte:
Adsorption
Analytical Chemistry
beta-Cyclodextrins - chemistry
Buffers
Chemistry
DNA - chemistry
gamma-Cyclodextrins - chemistry
Hydrogen-Ion Concentration
Lipopolysaccharides - chemistry
Lipopolysaccharides - isolation & purification
Microspheres
Osmolar Concentration
Solutions
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Zusammenfassung:The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 μg mL−1, pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL−1), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and
ISSN:0910-6340
1348-2246
1348-2246
DOI:10.2116/analsci.27.213