5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

Background No studies have evaluated the effectiveness of 5-ASA against oxidative DNA damage in experimental models of diversion colitis. Aim To evaluate the effects of 5-ASA against oxidative DNA damage in an experimental model of diversion colitis. Methods Twenty-six Wistar rats were divided into two groups corresponding to sacrifice at 2 or 4 weeks after fecal diversion of the left colon by means of proximal colostomy and distal mucosa fistula. Each group was divided into two subgroups according to intervention in excluded colon performed with 0.9% saline solution or 5-ASA. Level of oxidative DNA damage was determined by comet assay in cells obtained from segments with and without fecal stream before and after H 2 O 2 challenge. For statistical analysis, was used one-way analysis of variance (ANOVA), adopting a 5% significance level ( P