Fast and precise method for HPLC–size exclusion chromatography with UV and TOC (NDIR) detection: Importance of multiple detectors to evaluate the characteristics of dissolved organic matter
A new type of high-performance liquid chromatography (HPLC)–size exclusion chromatography (SEC) system with ultraviolet (UV) absorbance detection and non-dispersive infrared (NDIR) detection of total organic carbon is described. The introduction of an online degassing tube and a low-volume HPLC colu...
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Veröffentlicht in: | Water research (Oxford) 2011-11, Vol.45 (18), p.6240-6248 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A new type of high-performance liquid chromatography (HPLC)–size exclusion chromatography (SEC) system with ultraviolet (UV) absorbance detection and non-dispersive infrared (NDIR) detection of total organic carbon is described. The introduction of an online degassing tube and a low-volume HPLC column helped to reduce the analytical time and increase the sensitivity of the SEC system. This study is the first in which linear calibration curves (
R
2
>
0.99) were obtained for both UV absorbance and NDIR data for polystyrene sulfonate standards, which are the most suitable standards for molecular size analysis of aquatic humic substances as well as dissolved organic matter (DOM). Using the calibration curves, the molecular size distribution of DOM in water collected from Lake Kasumigaura and in pore water from lake sediments was estimated. Most of the DOM had a molecular weight less than 4000 Daltons (Da), and the amount of low-molecular-weight DOM (∼2000
Da) with low UV absorbance increased with depth in the sediment pore water. This result shows the importance of combining quantitative analysis by NDIR detection with qualitative analysis by UV detection to determine the chemical and physical properties of DOM. The possible sources and reactivity of DOM in Lake Kasumigaura and its sediment pore water are also discussed.
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► A fast and precise HPLC–SEC system with both UVA and TOC detectors was developed. ► The system achieved shorter analytical time and lower sample volume. ► HMW-DOM with low UVA and LMW-DOM comprised of DOM from Lake Kasumigaura. ► LMW-DOM could be provided from sediment pore water. |
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ISSN: | 0043-1354 1879-2448 |
DOI: | 10.1016/j.watres.2011.09.021 |