Shoot organogenesis and plant regeneration in Pityopsis ruthii

Shoot organogenesis and plant regeneration in Pityopsis ruthii

Pityopsis ruthii is an endangered herbaceous perennial species from the United States. In vitro multiplication of this species can be valuable for germplasm conservation. Flower receptacles of P. ruthii were cultured on Murashige and Skoog medium (MS) supplemented with 11.4 μM indole-3-acetic acid (...

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Veröffentlicht in:Plant cell, tissue and organ culture tissue and organ culture, 2011-09, Vol.106 (3), p.513-516
Hauptverfasser: Wadl, Phillip A., Dattilo, Adam J., Vito, Lisa M., Trigiano, Robert N.
Format: Artikel
Sprache:eng
Schlagworte:
Acetic acid
Benzyladenine
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Conservation
Endangered species
Explants
Flowers
Fundamental and applied biological sciences. Psychology
Genetic transformation
Germplasm
Growth regulators
Indoleacetic acid
Leaves
Life Sciences
Micropropagation
Multiplication
Organogenesis
Pityopsis ruthii
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Plant tissues
Plants (botany)
Receptacles
Regeneration
Research Note
Rooting
Shoots
Variance analysis
Wildlife conservation
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Zusammenfassung:Pityopsis ruthii is an endangered herbaceous perennial species from the United States. In vitro multiplication of this species can be valuable for germplasm conservation. Flower receptacles of P. ruthii were cultured on Murashige and Skoog medium (MS) supplemented with 11.4 μM indole-3-acetic acid (IAA) in combination with 2.2, 4.4 or 8.8 μM 6-benzyladenine (BA). Shoots were visible within 14–28 days and three plants were successfully rooted on MS medium supplemented with 5.7 μM IAA. A two tailed t-test for paired-variates revealed that shoot regeneration on MS medium amended with 11.4 μM IAA and 2.2 μM BA was significantly higher ( P  
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-011-9930-x