CAPS markers for the identification of garden pea (Pisum sativum L.) cultivars

The CAPS (PCR-RFLP) method was used to analyze polymorphism in sequences of unique genes among specimens of 24 pea lines and cultivars. Analysis of each employed molecular-genetic marker was found to reveal three to seven polymorphic sequence variants. Analysis with the use of five selected markers...

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Veröffentlicht in:Russian journal of genetics 2009-02, Vol.45 (2), p.251-254
Hauptverfasser: Konovalov, F. A, Toshchakova, E. V, Gostimsky, S. A
Format: Artikel
Sprache:eng
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Zusammenfassung:The CAPS (PCR-RFLP) method was used to analyze polymorphism in sequences of unique genes among specimens of 24 pea lines and cultivars. Analysis of each employed molecular-genetic marker was found to reveal three to seven polymorphic sequence variants. Analysis with the use of five selected markers allows the unambiguous identification of any of examined specimens. Thus, the possibility of using CAPS markers for identification and classification of garden pea cultivars has been shown. Possible prospects for this approach and the ways of its further implementation are considered.
ISSN:1022-7954
1608-3369
DOI:10.1134/S1022795409020197