Micronutrient optimization results into highly improved in vitro plant regeneration in kodo (Paspalum scrobiculatum L.) and finger (Eleusine coracana (L.) Gaertn.) millets

Basal medium constituents and their concentration play an important role in growth and morphogenesis of plant tissues cultured in vitro. In this study effect of different inorganic nutrients (CoCl₂, MnSO₄, ZnSO₄, CuSO₄ and AgNO₃) on callus induction and plant regeneration in Paspalum scrobiculatum and Eleusine coracana was examined. A 5x and 3x increase in regeneration response at enhanced levels of CuSO₄ was noted for kodo and finger millets, respectively. Significant improvement in plant regeneration was also observed with the increase in levels of Co and Mn. Addition of AgNO₃ to the basal medium also had a stimulatory effect on callus induction and plant regeneration. Optimization of nutrient level in the basal medium has highly significant role in obtaining maximum regeneration response from explants and callus culture.