Gene markers for grain polyphenol oxidase activity in common wheat

Polyphenol oxidase (PPO) in grain is regarded as a major factor resulting in time-dependent darkening of wheat end products, particularly for Asian noodles and steamed bread. Breeding wheat cultivars with low PPO activity using efficient and reliable markers is one of the best ways to reduce the und...

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Veröffentlicht in:	Molecular breeding 2009-01, Vol.23 (1), p.163-170
Hauptverfasser:	Wang, X. B, Ma, C. X, Si, H. Q, Qiao, Y. Q, Chang, C, He, X. F, Xia, Y. X
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Biomedical and Life Sciences Biotechnology Bread Cultivars Darkening Fragments Genotypes Grain Impact analysis Life Sciences Markers Medicare Molecular biology Noodles Plant biology Plant breeding Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polyphenol oxidase Time dependence Triticum aestivum Wheat
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Zusammenfassung:	Polyphenol oxidase (PPO) in grain is regarded as a major factor resulting in time-dependent darkening of wheat end products, particularly for Asian noodles and steamed bread. Breeding wheat cultivars with low PPO activity using efficient and reliable markers is one of the best ways to reduce the undesirable darkening. In the present study, we developed a gene-specific marker (PPO05) for low PPO activity from the sequence AY515506. This marker detected double PCR fragments (750 bp) in the cultivars with low PPO activity and a single PCR fragment (
ISSN:	1380-3743 1572-9788
DOI:	10.1007/s11032-008-9223-1