Soluble and immobilized phenol oxidase of the fungusMycelia sterilia IBR 35219/2: A comparative study

Soluble and immobilized phenol oxidase of the fungusMycelia sterilia IBR 35219/2: A comparative study

Phenol oxidase (EC 1.14.18.1) from the microscopic fungusMycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxid...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Applied biochemistry and microbiology 2000-03, Vol.36 (2), p.115-118
Hauptverfasser: Mchedlishvili, N I, Pruidze, G N, Omiadze, N T, Zukhbaya, R V
Format: Artikel
Sprache:eng
Schlagworte:
Acidic oxides
Comparative studies
Enzymes
Green tea
Immobilization
Oxidase
Phenolic compounds
Phenoloxidase
Phenols
Silica
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 118
container_issue 2
container_start_page 115
container_title Applied biochemistry and microbiology
container_volume 36
creator Mchedlishvili, N I
Pruidze, G N
Omiadze, N T
Zukhbaya, R V
description Phenol oxidase (EC 1.14.18.1) from the microscopic fungusMycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxidases were compared. Compared to the soluble enzyme, the activity of which was optimum at pH 5.5, immobilized phenol oxidase exhibited optimum activity under slightly more acidic conditions (pH 5.2). Immobilization considerably increased enzyme stability. Both soluble and immobilized forms of phenol oxidase fromM. sterilia IBR 35 219/2 catalyze oxidative conversion of phenolic compounds of green tea extract.
doi_str_mv 10.1007/BF02737904
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_902345804</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2171301231</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1634-39b9dd6c3a2bc565757a835a3cbf667e82194bd5206a3289d5bb9a65fb65ea323</originalsourceid><addsrcrecordid>eNp1kVtLwzAYhoMoOKc3_oKgF4JQlybNybttOB1MBA_XJWlTl9E2NWnF-evNUBAEr74DD9_3wAvAaYquUoT4ZLZAmBMuUbYHRilDIiEIZ_tghBAiCRNEHIKjEDZxlEzIETBPrh50baBqS2ibxmlb209Twm5tWldD92FLFQx0FezXBlZD-zqE-21haqtg6I23u2Y5e4SE4lRO8DWcwsI1nfKqt-8mMkO5PQYHlaqDOfmpY_CyuHme3yWrh9vlfLpKipSRLCFSy7JkBVFYF5RRTrkShCpS6IoxbkT8kOmSYsQUwUKWVGupGK00oyZuyBhcfN_tvHsbTOjzxoboWqvWuCHkEmGSUYGySJ79ITdu8G2UyzmVHDESjcbg_D8Ic8FoJpjcUZffVOFdCN5Ueedto_w2T1G-SyX_TYV8AYZ_fDI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>759706316</pqid></control><display><type>article</type><title>Soluble and immobilized phenol oxidase of the fungusMycelia sterilia IBR 35219/2: A comparative study</title><source>Springer Nature - Complete Springer Journals</source><creator>Mchedlishvili, N I ; Pruidze, G N ; Omiadze, N T ; Zukhbaya, R V</creator><creatorcontrib>Mchedlishvili, N I ; Pruidze, G N ; Omiadze, N T ; Zukhbaya, R V</creatorcontrib><description>Phenol oxidase (EC 1.14.18.1) from the microscopic fungusMycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxidases were compared. Compared to the soluble enzyme, the activity of which was optimum at pH 5.5, immobilized phenol oxidase exhibited optimum activity under slightly more acidic conditions (pH 5.2). Immobilization considerably increased enzyme stability. Both soluble and immobilized forms of phenol oxidase fromM. sterilia IBR 35 219/2 catalyze oxidative conversion of phenolic compounds of green tea extract.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1007/BF02737904</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>Acidic oxides ; Comparative studies ; Enzymes ; Green tea ; Immobilization ; Oxidase ; Phenolic compounds ; Phenoloxidase ; Phenols ; Silica</subject><ispartof>Applied biochemistry and microbiology, 2000-03, Vol.36 (2), p.115-118</ispartof><rights>MAIK “Nauka/Interperiodica” 2000.</rights><rights>Plenum Publishing Corporation 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1634-39b9dd6c3a2bc565757a835a3cbf667e82194bd5206a3289d5bb9a65fb65ea323</citedby><cites>FETCH-LOGICAL-c1634-39b9dd6c3a2bc565757a835a3cbf667e82194bd5206a3289d5bb9a65fb65ea323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Mchedlishvili, N I</creatorcontrib><creatorcontrib>Pruidze, G N</creatorcontrib><creatorcontrib>Omiadze, N T</creatorcontrib><creatorcontrib>Zukhbaya, R V</creatorcontrib><title>Soluble and immobilized phenol oxidase of the fungusMycelia sterilia IBR 35219/2: A comparative study</title><title>Applied biochemistry and microbiology</title><description>Phenol oxidase (EC 1.14.18.1) from the microscopic fungusMycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxidases were compared. Compared to the soluble enzyme, the activity of which was optimum at pH 5.5, immobilized phenol oxidase exhibited optimum activity under slightly more acidic conditions (pH 5.2). Immobilization considerably increased enzyme stability. Both soluble and immobilized forms of phenol oxidase fromM. sterilia IBR 35 219/2 catalyze oxidative conversion of phenolic compounds of green tea extract.</description><subject>Acidic oxides</subject><subject>Comparative studies</subject><subject>Enzymes</subject><subject>Green tea</subject><subject>Immobilization</subject><subject>Oxidase</subject><subject>Phenolic compounds</subject><subject>Phenoloxidase</subject><subject>Phenols</subject><subject>Silica</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kVtLwzAYhoMoOKc3_oKgF4JQlybNybttOB1MBA_XJWlTl9E2NWnF-evNUBAEr74DD9_3wAvAaYquUoT4ZLZAmBMuUbYHRilDIiEIZ_tghBAiCRNEHIKjEDZxlEzIETBPrh50baBqS2ibxmlb209Twm5tWldD92FLFQx0FezXBlZD-zqE-21haqtg6I23u2Y5e4SE4lRO8DWcwsI1nfKqt-8mMkO5PQYHlaqDOfmpY_CyuHme3yWrh9vlfLpKipSRLCFSy7JkBVFYF5RRTrkShCpS6IoxbkT8kOmSYsQUwUKWVGupGK00oyZuyBhcfN_tvHsbTOjzxoboWqvWuCHkEmGSUYGySJ79ITdu8G2UyzmVHDESjcbg_D8Ic8FoJpjcUZffVOFdCN5Ueedto_w2T1G-SyX_TYV8AYZ_fDI</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Mchedlishvili, N I</creator><creator>Pruidze, G N</creator><creator>Omiadze, N T</creator><creator>Zukhbaya, R V</creator><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20000301</creationdate><title>Soluble and immobilized phenol oxidase of the fungusMycelia sterilia IBR 35219/2: A comparative study</title><author>Mchedlishvili, N I ; Pruidze, G N ; Omiadze, N T ; Zukhbaya, R V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1634-39b9dd6c3a2bc565757a835a3cbf667e82194bd5206a3289d5bb9a65fb65ea323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acidic oxides</topic><topic>Comparative studies</topic><topic>Enzymes</topic><topic>Green tea</topic><topic>Immobilization</topic><topic>Oxidase</topic><topic>Phenolic compounds</topic><topic>Phenoloxidase</topic><topic>Phenols</topic><topic>Silica</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mchedlishvili, N I</creatorcontrib><creatorcontrib>Pruidze, G N</creatorcontrib><creatorcontrib>Omiadze, N T</creatorcontrib><creatorcontrib>Zukhbaya, R V</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mchedlishvili, N I</au><au>Pruidze, G N</au><au>Omiadze, N T</au><au>Zukhbaya, R V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Soluble and immobilized phenol oxidase of the fungusMycelia sterilia IBR 35219/2: A comparative study</atitle><jtitle>Applied biochemistry and microbiology</jtitle><date>2000-03-01</date><risdate>2000</risdate><volume>36</volume><issue>2</issue><spage>115</spage><epage>118</epage><pages>115-118</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Phenol oxidase (EC 1.14.18.1) from the microscopic fungusMycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxidases were compared. Compared to the soluble enzyme, the activity of which was optimum at pH 5.5, immobilized phenol oxidase exhibited optimum activity under slightly more acidic conditions (pH 5.2). Immobilization considerably increased enzyme stability. Both soluble and immobilized forms of phenol oxidase fromM. sterilia IBR 35 219/2 catalyze oxidative conversion of phenolic compounds of green tea extract.</abstract><cop>Dordrecht</cop><pub>Springer Nature B.V</pub><doi>10.1007/BF02737904</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0003-6838
ispartof Applied biochemistry and microbiology, 2000-03, Vol.36 (2), p.115-118
issn 0003-6838
1608-3024
language eng
recordid cdi_proquest_miscellaneous_902345804
source Springer Nature - Complete Springer Journals
subjects Acidic oxides
Comparative studies
Enzymes
Green tea
Immobilization
Oxidase
Phenolic compounds
Phenoloxidase
Phenols
Silica
title Soluble and immobilized phenol oxidase of the fungusMycelia sterilia IBR 35219/2: A comparative study
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T16%3A12%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Soluble%20and%20immobilized%20phenol%20oxidase%20of%20the%20fungusMycelia%20sterilia%20IBR%2035219/2:%20A%20comparative%20study&rft.jtitle=Applied%20biochemistry%20and%20microbiology&rft.au=Mchedlishvili,%20N%20I&rft.date=2000-03-01&rft.volume=36&rft.issue=2&rft.spage=115&rft.epage=118&rft.pages=115-118&rft.issn=0003-6838&rft.eissn=1608-3024&rft_id=info:doi/10.1007/BF02737904&rft_dat=%3Cproquest_cross%3E2171301231%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=759706316&rft_id=info:pmid/&rfr_iscdi=true