Soluble and immobilized phenol oxidase of the fungusMycelia sterilia IBR 35219/2: A comparative study

Phenol oxidase (EC 1.14.18.1) from the microscopic fungusMycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxid...

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Veröffentlicht in:	Applied biochemistry and microbiology 2000-03, Vol.36 (2), p.115-118
Hauptverfasser:	Mchedlishvili, N I, Pruidze, G N, Omiadze, N T, Zukhbaya, R V
Format:	Artikel
Sprache:	eng
Schlagworte:	Acidic oxides Comparative studies Enzymes Green tea Immobilization Oxidase Phenolic compounds Phenoloxidase Phenols Silica
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Zusammenfassung:	Phenol oxidase (EC 1.14.18.1) from the microscopic fungusMycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxidases were compared. Compared to the soluble enzyme, the activity of which was optimum at pH 5.5, immobilized phenol oxidase exhibited optimum activity under slightly more acidic conditions (pH 5.2). Immobilization considerably increased enzyme stability. Both soluble and immobilized forms of phenol oxidase fromM. sterilia IBR 35 219/2 catalyze oxidative conversion of phenolic compounds of green tea extract.
ISSN:	0003-6838 1608-3024
DOI:	10.1007/BF02737904