Immobilization of invertase on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole

Immobilization of invertase on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole

In this study, immobilization of invertase was achieved on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole) (SNS (NO2)) via electrochemical polymerization. Kinetic parameters, maximum reaction rate (Vmax) and substrate affinity (Km), optimum temperature and pH, operational and...

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Veröffentlicht in:Reactive & functional polymers 2008-03, Vol.68 (3), p.710-717
Hauptverfasser: Tuncagil, Sevinc, Kiralp, Senem, Varis, Serhat, Toppare, Levent
Format: Artikel
Sprache:eng
Schlagworte:
Affinity
Conducting polymers
Enzymes
Immobilization
Invertase
Optimization
Polymers
Storage stability
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Zusammenfassung:In this study, immobilization of invertase was achieved on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole) (SNS (NO2)) via electrochemical polymerization. Kinetic parameters, maximum reaction rate (Vmax) and substrate affinity (Km), optimum temperature and pH, operational and storage stabilities of immobilized enzyme were determined.
ISSN:1381-5148
DOI:10.1016/j.reactfunctpolym.2007.11.010