Characterization of Ultra-Thin Temperature-Responsive Polymer Layer and Its Polymer Thickness Dependency on Cell Attachment/Detachment Properties

Ultra thin poly(N‐isopropylacrylamide) (PIPAAm) modified glass coverslips (PIAPAm‐CS) using electron beam irradiation exhibited a clear relationship between the polymer thickness and thermal cell adhesion/detachment behavior. The polymer thickness dependency and the characteristic of ultra thin PIPAAm layer, has been illustrated in terms of the molecular motion of the modified PIPAAm chains. PIPAAm‐CSs surfaces with various area‐polymer densities and thicknesses were characterized by AFM and protein adsorption assay. The newly obtained results gave a further insight into the illustration. Finally, the future application of intelligent surfaces was discussed for fabricating tissue and organ. Ultra thin poly(N‐isopropylacrylamide) (PIPAAm) modified surfaces using electron beam irradiation exhibited the polymer thickness dependency on following cell adhesion/detachment in response to temperature change. The various thicknesses of PIPAAm modified surfaces ware characterized, and the polymer thickness dependency on glass and polystyrene was discussed in terms of dynamics of the polymer chains.