High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an in...

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Veröffentlicht in:Analyst (London) 2011-11, Vol.136 (22), p.4670-4673
Hauptverfasser: KNEZEVIC, Ana, BONES, Jonathan, STJEPAN KRESIMIR KRACUN, GORNIK, Olga, RUDD, Pauline M, LAUC, Gordan
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Aniline
Chemistry
Chromatography, High Pressure Liquid - methods
Exact sciences and technology
Fluorescence
Glycomics - methods
Humans
Labelling
Multiplexing
Polysaccharides - blood
Profiling
Spectrometric and optical methods
Spectrometry, Fluorescence - methods
Staining and Labeling - methods
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Beschreibung
Zusammenfassung:A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.
ISSN:0003-2654
1364-5528
DOI:10.1039/c1an15684e