Identification of novel mammalian phospholipids containing threonine, aspartate, and glutamate as the base moiety

In this study, we showed the occurrence of phosphatidyl- l-threonine (PThr), phosphatidyl- l-aspartate (PAsp), and phosphatidyl- l-glutamate (PGlu) in rat brain. Analyses using an HPLC–ESI-MS and an amino acid analyzer showed the presence of l-threonine, l-aspartate, and l-glutamate in the acid-hydr...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-11, Vol.879 (29), p.3296-3302
Hauptverfasser:	Omori, Taketo, Honda, Ai, Mihara, Hisaaki, Kurihara, Tatsuo, Esaki, Nobuyoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aspartate aspartic acid Aspartic Acid - analogs & derivatives Aspartic Acid - chemistry Carboxylate–phosphate anhydride cerebrum Cerebrum - chemistry chromatography Chromatography, High Pressure Liquid - methods Glutamate glutamic acid Glutamic Acid - analogs & derivatives Glutamic Acid - chemistry Glycerophospholipids - chemistry liver Liver - chemistry Mammal Phospholipids Rats Spectrometry, Mass, Electrospray Ionization - methods Swine Tandem Mass Spectrometry Threonine Threonine - analogs & derivatives Threonine - chemistry
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Zusammenfassung:	In this study, we showed the occurrence of phosphatidyl- l-threonine (PThr), phosphatidyl- l-aspartate (PAsp), and phosphatidyl- l-glutamate (PGlu) in rat brain. Analyses using an HPLC–ESI-MS and an amino acid analyzer showed the presence of l-threonine, l-aspartate, and l-glutamate in the acid-hydrolysates of phospholipids from porcine cerebrum, rat cerebrum, and rat liver. Results of ESI-MS/MS analyses with neutral loss scanning and product ion scanning suggest the presence of PThr-(18:0, 18:1), PThr-(18:0, 22:6), PAsp-(18:0, 18:1), PAsp-(18:0, 22:6), PGlu-(18:0, 18:1), and PGlu-(18:0, 22:6) in rat brain. This is the first study to identify 2 novel phospholipids, PAsp and PGlu, with a carboxylate–phosphate anhydride bond, in living organisms.
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2011.04.033