Diagnostic utility of combination of inducer and inhibitor based assay in detection of Pseudomonas aeruginosa producing AmpC β-lactamase

The diagnostic utility of inducer and inhibitor based assays among 214 AmpC positive isolates of Pseudomonas aeruginosa were evaluated. Of different methods, combination of ceftazidime–imipenem antagonism and boronic acid inhibition tests came up with maximum sensitivity (76%) and specificity (100%)...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2011-10, Vol.87 (1), p.116-118
Hauptverfasser:	Upadhyay, Supriya, Sen, Malay Ranjan, Bhattacharjee, Amitabha, Prakash, Pradyot, Bajpai, Ram Chandra, Anupurba, Shampa
Format:	Artikel
Sprache:	eng
Schlagworte:	AmpC β-lactamases Anti-Bacterial Agents - pharmacology Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriological methods and techniques used in bacteriology Bacteriology beta-lactamase beta-Lactamases - genetics beta-Lactamases - metabolism Biological and medical sciences boron compounds Boronic acid Boronic Acids - pharmacology ceftazidime Cloxacillin - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - drug effects Genotype Humans imipenem Inducer Inhibitor Microbial Sensitivity Tests - methods Microbiology P. aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa - classification Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - genetics Pseudomonas Infections - diagnosis Pseudomonas Infections - microbiology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The diagnostic utility of inducer and inhibitor based assays among 214 AmpC positive isolates of Pseudomonas aeruginosa were evaluated. Of different methods, combination of ceftazidime–imipenem antagonism and boronic acid inhibition tests came up with maximum sensitivity (76%) and specificity (100%). This combination showed reliability for both inducible and non-inducible AmpC producers. ► We evaluated the combination of inducer and inhibitor based method for AmpC detection in P. aeruginosa. ► Ceftazidime imipenem antagonism test proved to be better, detecting 24.6% inducible AmpC producers. ► Of inhibitors, boronic acid was more effectively detected AmpC in 30.7% isolates. ► We demonstrated that combination of CIAT along with boronic acid inhibition test could detect maximum AmpC producers. ► CIAT along with boronic acid inhibition test yielded maximum sensitivity (85%) and specificity (100%).
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2011.07.012