The influence of growth conditions on the cell dry weight per unit biovolume of Klebsormidium flaccidum (Charophyta), a typical ubiquitous soil alga

The dry weight per unit biovolume of 810 single, living cells of the ubiquitous soil algae Klebsormidium flaccidum from 80 experiments were determined using a Mach–Zehnder double-beam interference microscope. Different substrates such as agarized nutrient solution, different soils, and slag heap material, different pH values, temperatures and light intensities were used and cells from both growth and stationary phase were measured. The total possible range of dry weight with respect to carbon per unit biovolume (C/ubv) values was 93–226 fg μm −3 . The mean value of all data was 147 fg μm −3 , which concurs with the average value as taken from literature data of several planktonic algal species and groups within the respective size range (cell volume 300–1,000 μm 3 ). We could show that C/ubv is suitable to quantify environmental stress conditions: C/ubv values ≤140 fg μm −3 are characteristic of cells grown under optimum conditions, and values ≥160 fg μm −3 reflect quantitatively graded stress situations. We propose integrating the microscope interferometric method using K. flaccidum as a test organism into a soil test system to determine the prevailing environmental conditions.