Gonadotropins in the Manchurian trout, Brachymystax lenok tsinlingensis

Gonadotropins in the Manchurian trout, Brachymystax lenok tsinlingensis

cDNA clones encoding gonadotropin (GTH) α, follicle-stimulating hormone (FSH) β and luteinzing hormone (LH) β were isolated from the pituitaries of maturing Manchurian trout (Brachymystax lenok tsinlingensis) and sequenced. The deduced amino acid sequences of GTH subunits showed high identities to m...

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Veröffentlicht in:Fish physiology and biochemistry 2003-01, Vol.28 (1-4), p.89-90
Hauptverfasser: Choi, E.J., Jin, D.H., Sohn, Y.C.
Format: Artikel
Sprache:eng
Schlagworte:
Amino acids
Brachymystax lenok
Eucarya
Oncorhynchus masou
Salmon
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Zusammenfassung:cDNA clones encoding gonadotropin (GTH) α, follicle-stimulating hormone (FSH) β and luteinzing hormone (LH) β were isolated from the pituitaries of maturing Manchurian trout (Brachymystax lenok tsinlingensis) and sequenced. The deduced amino acid sequences of GTH subunits showed high identities to masu salmon, Oncorhynchus masou: GTHα1 (95%), FSHβ (92%) and LHβ (97%), respectively. We are also attempting to produce recombinant FSH and LH using a eukaryotic expression system. In a pilot experiment, LH was secreted to the culture medium at 48 and 60 hrs after transfection. The results will be helpful to develop controlled reproduction of exterminating Manchurian trout.[PUBLICATION ABSTRACT]
ISSN:0920-1742
1573-5168
DOI:10.1023/B:FISH.0000030483.11026.e5