Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

A multiplex PCR assay was developed based on atpA-sequence diversification for molecular identification of 3 major pathogenic Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. It specifically identified them from among 133 strains of various Vibrio species and other ge...

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Veröffentlicht in:	Molecular and cellular probes 2011-08, Vol.25 (4), p.174-176
Hauptverfasser:	Izumiya, Hidemasa, Matsumoto, Kazutoshi, Yahiro, Shunsuke, Lee, Jiyoung, Morita, Masatomo, Yamamoto, Shouji, Arakawa, Eiji, Ohnishi, Makoto
Format:	Artikel
Sprache:	eng
Schlagworte:	atpA Bacterial Typing Techniques - methods Multiplex PCR Phylogeny Polymerase Chain Reaction - methods V. cholerae V. parahaemolyticus V. vulnificus Vibrio - classification Vibrio - genetics Vibrio - isolation & purification Vibrio cholerae Vibrio cholerae - classification Vibrio cholerae - genetics Vibrio cholerae - isolation & purification Vibrio parahaemolyticus Vibrio parahaemolyticus - classification Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - isolation & purification Vibrio vulnificus Vibrio vulnificus - classification Vibrio vulnificus - genetics Vibrio vulnificus - isolation & purification
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Beschreibung
Zusammenfassung:	A multiplex PCR assay was developed based on atpA-sequence diversification for molecular identification of 3 major pathogenic Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. It specifically identified them from among 133 strains of various Vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness.
ISSN:	0890-8508 1096-1194
DOI:	10.1016/j.mcp.2011.04.004