Halomonas stenophila sp. nov., a halophilic bacterium that produces sulphate exopolysaccharides with biological activity

We have undertaken a polyphasic taxonomic study of two halophilic, Gram-negative bacterial strains, N12T and B-100, that produce sulphated exopolysaccharides with biological activity. They were isolated from two different saline soil samples. Both strains grow at NaCl concentrations within the range 3–15 % (w/v) [optimum 5–10 % (w/v)], at 15–37 °C (optimum 20–32 °C) and at pH 6–8 (optimum pH 7–8). Their 16S rRNA gene sequences indicate that they belong to the genus Halomonas in the class GAMMAPROTEOBACTERIA: Their closest relative is Halomonas nitroreducens, to which our strains show maximum 16S rRNA gene sequence similarity values of 98.7 % (N12T) and 98.3 % (B-100). Their DNA G+C contents are 61.9 and 63.8 mol%, respectively. The results of DNA–DNA hybridizations showed 43.9 % relatedness between strain N12T and H. nitroreducens CECT 7281T, 30.5 % between N12T and Halomonas ventosae CECT 5797T, 39.2 % between N12T and Halomonas fontilapidosi CECT 7341T, 46.3 % between N12T and Halomonas maura CECT 5298T, 52.9 % between N12T and Halomonas saccharevitans LMG 23976T, 51.3 % between N12T and Halomonas koreensis JCM 12237T and 100 % between strains N12T and B-100. The major fatty acids of strain N12T are C12 : 0 3-OH (5.42 %), C15 : 0 iso 2-OH/C16 : 1ω7c (17.37 %), C16 : 0 (21.62 %) and C18 : 1ω7c (49.19 %). The proposed name for the novel species is Halomonas stenophila sp. nov. Strain N12T ( = CECT 7744T = LMG 25812T) is the type strain.