Two-Photon Lysotrackers for in Vivo Imaging

We report two-photon Lysotrackers (CLT-blue and CLT-yellow) that can be excited by 750–840 nm femtosecond laser pulses and emit at 470 and 550 nm, respectively. They can be easily loaded into cells and tissue slices for visualization of lysosomes in live cells and tissues for a long period of time t...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of organic chemistry 2011-10, Vol.76 (19), p.8113-8116
Hauptverfasser:	Son, Ji Hoon, Lim, Chang Su, Han, Ji Hee, Danish, Isravel Antony, Kim, Hwan Myung, Cho, Bong Rae
Format:	Artikel
Sprache:	eng
Schlagworte:	Amines - analysis Amines - chemical synthesis Amines - chemistry Animals Chemistry Exact sciences and technology Fluorescent Dyes - analysis Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Heterocyclic compounds Heterocyclic compounds with o, s, se, te hetero atom and condensed derivatives Hippocampus - cytology Molecular Imaging - methods Organic chemistry Photons Preparations and properties Rats
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We report two-photon Lysotrackers (CLT-blue and CLT-yellow) that can be excited by 750–840 nm femtosecond laser pulses and emit at 470 and 550 nm, respectively. They can be easily loaded into cells and tissue slices for visualization of lysosomes in live cells and tissues for a long period of time through two-photon microscopy. When combined with appropriate two-photon probes for other biological targets, these novel probes would greatly facilitate the two-photon microscopy colocalization experiments.
ISSN:	0022-3263 1520-6904
DOI:	10.1021/jo201504h