Oligonucleotide-based label-free Hg2+ assay with a monomer-excimer fluorescence switch

Oligonucleotide-based label-free Hg2+ assay with a monomer-excimer fluorescence switch

A novel fluorescent Hg(2+) sensor was developed based on the T-Hg(2+)-T structure and a thioflavine T monomer-excimer fluorescent switch. Under optimum conditions, the selectivity is remarkably high, and Hg(2+) can be quantified over the dynamic range of 0.1 to 1.2 μM, with a limit of detection (LOD...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analyst (London) 2011-10, Vol.136 (20), p.4284-4288
Hauptverfasser: Wang, Yongxiang, Geng, Fenghua, Cheng, Qianli, Xu, Huiying, Xu, Maotian
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Chemistry
Exact sciences and technology
General, instrumentation
Hydrogen-Ion Concentration
Mercury - analysis
Oligonucleotides - chemistry
Spectrometric and optical methods
Spectrometry, Fluorescence
Thiazoles - chemistry
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A novel fluorescent Hg(2+) sensor was developed based on the T-Hg(2+)-T structure and a thioflavine T monomer-excimer fluorescent switch. Under optimum conditions, the selectivity is remarkably high, and Hg(2+) can be quantified over the dynamic range of 0.1 to 1.2 μM, with a limit of detection (LOD) of ~20 nM and a linear correlation coefficient of 0.995.
ISSN:0003-2654
1364-5528
DOI:10.1039/c1an15465f