Signal amplification by substrate recycling on polyaniline/lactate oxidase/lactate dehydrogenase bienzyme electrodes

The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2001-10, Vol.96 (1/3), p.239-248
Hauptverfasser:	Chaubey, A, Pande, K.K, Pandey, M.K, Singh, V.S
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Aniline Compounds - chemistry Ascorbic Acid - chemistry Biochemistry Biological and medical sciences Biotechnology Crystals Dehydrogenase Dehydrogenases Dose-Response Relationship, Drug electrochemistry Electrodes Enzymes Fundamental and applied biological sciences. Psychology Glucose - chemistry Glutamic Acid - chemistry Hydrogen-Ion Concentration L-Lactate dehydrogenase L-Lactate Dehydrogenase - chemistry lactate 2-monooxygenase Lactate dehydrogenase Lactate oxidase Lactic acid Lactic Acid - chemistry Microscopy, Electron, Scanning Mixed Function Oxygenases - chemistry Models, Chemical Oxidase Polyanilines Recycling Signal transduction Studies Substrates Temperature Uric Acid - chemistry
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Zusammenfassung:	The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible to detect L-lactate at lower concentrations (0.1-1 mM). The PANI/LOD/LDH bienzyme electrodes were found to be stable for about 21 d at 4-10 degrees C.
ISSN:	0273-2289 1559-0291 0273-2289
DOI:	10.1385/ABAB:96:1-3:239