High-throughput quantification of hydroxyproline for determination of collagen

An accurate and high-throughput assay for collagen is essential for collagen research and development of collagen products. Hydroxyproline is routinely assayed to provide a measurement for collagen quantification. The time required for sample preparation using acid hydrolysis and neutralization prio...

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Veröffentlicht in:Analytical biochemistry 2011-10, Vol.417 (2), p.289-291
Hauptverfasser: Hofman, Kathleen, Hall, Bronwyn, Cleaver, Helen, Marshall, Susan
Format: Artikel
Sprache:eng
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Zusammenfassung:An accurate and high-throughput assay for collagen is essential for collagen research and development of collagen products. Hydroxyproline is routinely assayed to provide a measurement for collagen quantification. The time required for sample preparation using acid hydrolysis and neutralization prior to assay is what limits the current method for determining hydroxyproline. This work describes the conditions of alkali hydrolysis that, when combined with the colorimetric assay defined by Woessner, provide a high-throughput, accurate method for the measurement of hydroxyproline.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2011.06.019