Blood Plasma Sample Preparation Method for the Assessment of Thyroid Hormone-Disrupting Potency in Effect-Directed Analysis

A sample preparation method combining solid-phase extraction (SPE) and liquid–liquid extraction (LLE) was developed to be used in Effect-Directed Analysis (EDA) of blood plasma. Until now such a method was not available. It can be used for extraction of a broad range of thyroid hormone (TH)-disrupto...

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Veröffentlicht in:	Environmental science & technology 2011-09, Vol.45 (18), p.7936-7944
Hauptverfasser:	Simon, Eszter, Bytingsvik, Jenny, Jonker, Willem, Leonards, Pim E. G, de Boer, Jacob, Jenssen, Bjørn M, Lie, Elisabeth, Aars, Jon, Hamers, Timo, Lamoree, Marja H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal, plant and microbial ecology Animals Applied ecology Binding sites Binding, Competitive Bioassays Biological and medical sciences Cattle - blood Chemical compounds Ecotoxicology and Human Environmental Health Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on vertebrates Endocrine Disruptors - analysis Endocrine Disruptors - metabolism Extraction processes Female Fluorocarbons - analysis Fluorocarbons - metabolism Fundamental and applied biological sciences. Psychology Halogenated Diphenyl Ethers - analysis Halogenated Diphenyl Ethers - metabolism Hormones Mammalia PCB Phenols - analysis Phenols - metabolism Plasma Plasma - chemistry Polychlorinated biphenyls Polychlorinated Biphenyls - analysis Polychlorinated Biphenyls - metabolism Reproducibility of Results Solid Phase Extraction Thyroid gland Thyroxine - metabolism Thyroxine-Binding Proteins - metabolism Ursidae - blood
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Beschreibung
Zusammenfassung:	A sample preparation method combining solid-phase extraction (SPE) and liquid–liquid extraction (LLE) was developed to be used in Effect-Directed Analysis (EDA) of blood plasma. Until now such a method was not available. It can be used for extraction of a broad range of thyroid hormone (TH)-disruptors from plasma with high recoveries. Validation of the method using spiked cow plasma showed good recoveries for hydroxylated polybrominated diphenyl ethers (OH-PBDEs; 93.8 ± 19.5%), hydroxylated polychlorinated biphenyls (OH-PCBs; 93.8 ± 15.5%), other halogenated phenols (OHPs; 107 ± 8.1%), and for short-chain (
ISSN:	0013-936X 1520-5851
DOI:	10.1021/es2016389