Full sequence analysis of the original Sapporo virus

ABSTRACT In this study, the full‐length genome sequence of the prototype of sapovirus, namely Sapporo virus (SV82), was identified. Sapporo virus RNA was extracted from a fecal sample, amplified by RT‐PCR and the PCR products sequenced directly and analyzed. Sequence analysis showed that Sapporo vir...

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Veröffentlicht in:	Microbiology and immunology 2011-09, Vol.55 (9), p.657-660
Hauptverfasser:	Nakanishi, Kaori, Tatsumi, Masatoshi, Kinoshita-Numata, Kazuko, Tsugawa, Takeshi, Nakata, Shuji, Tsutsumi, Hiroyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	acute gastroenteritis Cluster Analysis Feces - virology Genome, Viral Humans Molecular Sequence Data Open Reading Frames Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics Sapovirus - genetics Sapovirus - isolation & purification sapovirus SV82 Sapporo virus Sequence Analysis, DNA Sequence Homology, Nucleic Acid
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Beschreibung
Zusammenfassung:	ABSTRACT In this study, the full‐length genome sequence of the prototype of sapovirus, namely Sapporo virus (SV82), was identified. Sapporo virus RNA was extracted from a fecal sample, amplified by RT‐PCR and the PCR products sequenced directly and analyzed. Sequence analysis showed that Sapporo virus consists of 7433 nucleotides and has three open reading frames. The Sapporo strain shows 91.7% nucleotide sequence identity to the Manchester virus. Phylogenic analysis has also revealed the closeness of Sapporo virus to other sapovirus/genogroup I strains. Basic information on the evolutionary history of sapovirus analysis is provided here.
ISSN:	0385-5600 1348-0421
DOI:	10.1111/j.1348-0421.2011.00358.x