Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells

Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells

An laser is used to produce DNA damage in rat kangaroo and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of H2AX, Nbs1, and Rad50 to the site of irradiation 3 to following laser exposur...

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Veröffentlicht in:Journal of Biomedical Optics 2007-03, Vol.12 (2), p.020505-020503
Hauptverfasser: Gomez-Godinez, Veronica, Wakida, Nicole M, Dvornikov, Alexander S, Yokomori, Kyoko, Berns, Michael W
Format: Artikel
Sprache:eng
Schlagworte:
DNA - chemistry
DNA - genetics
DNA - radiation effects
DNA Damage
DNA-Binding Proteins - genetics
Dose-Response Relationship, Radiation
femtosecond laser
Infrared Rays
laser microsurgery
Lasers
Radiation Dosage
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Zusammenfassung:An laser is used to produce DNA damage in rat kangaroo and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of H2AX, Nbs1, and Rad50 to the site of irradiation 3 to following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.2717684