Tuning DNA Stability To Achieve Turnover in Template for an Enzymatic Ligation Reaction

Learning to let go: Introducing destabilizing modifications into a DNA template leads to turnover in a DNA‐templated ligation reaction. By incorporating a cross‐catalytic cycle, self‐replication was also achieved, with one target able to make 32 copies of itself (see picture). This destabilization approach represents a general method for incorporating amplification into DNA ligation processes using T4 DNA ligase.