RAPD analysis for genetic diversity of Varanus salvator

RAPD analysis for genetic diversity of Varanus salvator

[Objective] The aim was to carry out RAPD analysis on genetic diversity of Varanus salvator. [Method] 20 random primers were used for PCR amplification of genomic DNA of 36 individuals of V. salvator. [Result] 10 primers could produce highly reproducible RAPD bands. A total of 2 952 DNA fragments we...

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Veröffentlicht in:Hunan agricultural science & technology newsletter : HASTN 2011-03, Vol.12 (3), p.400-404
Hauptverfasser: Fu Meilan, Ji’nan University, Guangzhou(China)，Life Science and Technology Institute, Yu Dongmei, South China Institute of Endangered Animals, Guangzhou (China), Wang Ying, Ji’nan University, Guangzhou(China)，Life Science and Technology Institute
Format: Artikel
Sprache:eng
Schlagworte:
BIODIVERSIDAD
BIODIVERSITE
BIODIVERSITY
DNA
Evolution
Genetic distance
Genetic diversity
GENETIC RESOURCES
genomics
http://www.fao.org/aos/agrovoc#c_3218
http://www.fao.org/aos/agrovoc#c_33949
http://www.fao.org/aos/agrovoc#c_36341
http://www.fao.org/aos/agrovoc#c_4404
LAGARTO
LEZARD
LIZARDS
Phylogeny
Polymerase chain reaction
Population differentiation
Primers
Random amplified polymorphic DNA
RAPD
RECURSOS GENETICOS
RESSOURCE GENETIQUE
Trees
Varanus salvator
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Zusammenfassung:[Objective] The aim was to carry out RAPD analysis on genetic diversity of Varanus salvator. [Method] 20 random primers were used for PCR amplification of genomic DNA of 36 individuals of V. salvator. [Result] 10 primers could produce highly reproducible RAPD bands. A total of 2 952 DNA fragments were successfully amplified. Each individual got 82 amplified bands on average, 47 of which showed polymorphism. The polymorphic locus percent was 57.32%. The genetic distance among 36 individuals ranged from 0.035 9 to 0.335 9 with an average of 0.135 9. The Nei’s gene diversity index (H) and Shannon’s information index (I) were 0.1819 and 0.2630, respectively, indicating that V. salvator had greater genetic diversity. [Conclusion] The phylogenetic tree was inferred by using UPGMA analysis, it was found that the 36 individuals could be classified as one group, and there was no obvious population differentiation.
ISSN:1009-4229