Magnetic separation of cells from developing embryoid bodies using magnetite cationic liposomes

Magnetic separation of cells from developing embryoid bodies using magnetite cationic liposomes

Embryoid bodies resemble post-implantation egg-cylinder stage embryos and are used to differentiate embryonic stem cells in vitro. In this study, we enriched mouse vasa homolog-positive germ cells from embryoid bodies after 8 d of differentiation using a magnetic separation method with magnetite cat...

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Veröffentlicht in:Journal of bioscience and bioengineering 2011-08, Vol.112 (2), p.184-187
Hauptverfasser: Horie, Masanobu, Ito, Akira, Maki, Takeshi, Kawabe, Yoshinori, Kamihira, Masamichi
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Biotechnology
Cations
Cell Differentiation
Cell Line
Cell Separation - methods
Cells, Cultured
Embryoid Bodies - cytology
Embryoid body
Embryonic stem cells
Embryonic Stem Cells - cytology
Ferrosoferric Oxide - chemistry
Fundamental and applied biological sciences. Psychology
Germ cells
Germ Cells - cytology
Liposomes - chemistry
Magnetic force
Magnetic separation
Mice
Mice, Inbred BALB C
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Zusammenfassung:Embryoid bodies resemble post-implantation egg-cylinder stage embryos and are used to differentiate embryonic stem cells in vitro. In this study, we enriched mouse vasa homolog-positive germ cells from embryoid bodies after 8 d of differentiation using a magnetic separation method with magnetite cationic liposomes.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2011.04.011