Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442
The PhaF is a nucleoid-associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; the N-terminal PHA granule-binding domain (phasin domain) and the C-terminal half containing AAKP-like tandem repeats ch...
Gespeichert in:
Veröffentlicht in: | Molecular microbiology 2011, Vol.79 (2), p.402-418 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 418 |
---|---|
container_issue | 2 |
container_start_page | 402 |
container_title | Molecular microbiology |
container_volume | 79 |
creator | Galán, B Dinjaski, N Maestro, B de Eugenio, L.I Escapa, I.F Sanz, J.M García, J.L Prieto, M.A |
description | The PhaF is a nucleoid-associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; the N-terminal PHA granule-binding domain (phasin domain) and the C-terminal half containing AAKP-like tandem repeats characteristic of the histone H1 family. Although the PhaF binding to PHA granules and its role as transcriptional regulator have been previously demonstrated, the cell physiology meaning of these properties remains unknown. This work demonstrates that PhaF plays a crucial role in granule localization within the cell. TEM and flow cytometry studies of cells producing granules at early growth stage demonstrated that PhaF directs the PHA granules to the centre of the cells, forming a characteristic needle array. Our studies demonstrated the existence of two markedly different cell populations in the strain lacking PhaF protein, i.e. cells with and without PHA. Complementation studies definitively demonstrated a key role of PhaF in granule segregation during the cell division ensuring the equal distribution of granules between daughter cells. In vitro studies showed that PhaF binds DNA through its C-terminal domain in a non-specific manner. All these findings suggested a main role of PhaF in PHA apparatus through interactions with the segregating chromosome. |
doi_str_mv | 10.1111/j.1365-2958.2010.07450.x |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_888096729</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>837453926</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5310-9f978b8e451e2c8de4bd2e917e334f636e3a8b8e1cdfc51a6e4c15bdb775278e3</originalsourceid><addsrcrecordid>eNqNkstu1DAUhiMEotPCK4CFhFhl8C2JvWCBKgoVLVSildhZZ2xnxoMnDvaEzjwDL43TDEViA9749v3H55zfRYEInpM8Xq_nhNVVSWUl5hTnU9zwCs93D4rZ_cXDYoZlhUsm6Nej4jilNcaE4Zo9Lo4ooUTyGs-Kn58G7W1wpoSUgnawtQZdreAM9StIrkMmuh82IddtI2jr_eAhIh80bF3oEHQGJbuMdjntQ4v64PervYlhtwf_DbqQQ6JlhG7wd3HQVbKDCZvQQUL9sHUG0Mdryjl9UjxqwSf79DCfFDdn765PP5QXn9-fn769KHXFCC5lKxuxEJZXxFItjOULQ60kjWWMtzWrLYPxnmjT6opAbbkm1cIsmqaijbDspHg1xe1j-D7YtFUbl8baoLNhSEoIgWXdUPlvkuW-M0nrTL74i1yHIXa5DCV4LVnDBM-QmCAdQ0rRtqqPbgNxrwhWo7FqrUb_1OifGo1Vd8aqXZY-O8QfFhtr7oW_nczAywMASYNvc8O1S384NtbDx0TfTNyt83b_3wmoy8vzcZX1zyd9C0HBMuY3br7Q8WcRmd0Rkv0CpVzJvA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>846937384</pqid></control><display><type>article</type><title>Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442</title><source>Wiley-Blackwell Journals</source><source>Wiley-Blackwell Open Access Backfiles</source><source>MEDLINE</source><source>EZB Electronic Journals Library</source><creator>Galán, B ; Dinjaski, N ; Maestro, B ; de Eugenio, L.I ; Escapa, I.F ; Sanz, J.M ; García, J.L ; Prieto, M.A</creator><creatorcontrib>Galán, B ; Dinjaski, N ; Maestro, B ; de Eugenio, L.I ; Escapa, I.F ; Sanz, J.M ; García, J.L ; Prieto, M.A</creatorcontrib><description>The PhaF is a nucleoid-associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; the N-terminal PHA granule-binding domain (phasin domain) and the C-terminal half containing AAKP-like tandem repeats characteristic of the histone H1 family. Although the PhaF binding to PHA granules and its role as transcriptional regulator have been previously demonstrated, the cell physiology meaning of these properties remains unknown. This work demonstrates that PhaF plays a crucial role in granule localization within the cell. TEM and flow cytometry studies of cells producing granules at early growth stage demonstrated that PhaF directs the PHA granules to the centre of the cells, forming a characteristic needle array. Our studies demonstrated the existence of two markedly different cell populations in the strain lacking PhaF protein, i.e. cells with and without PHA. Complementation studies definitively demonstrated a key role of PhaF in granule segregation during the cell division ensuring the equal distribution of granules between daughter cells. In vitro studies showed that PhaF binds DNA through its C-terminal domain in a non-specific manner. All these findings suggested a main role of PhaF in PHA apparatus through interactions with the segregating chromosome.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2010.07450.x</identifier><identifier>PMID: 21219460</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Binding sites ; Biological and medical sciences ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cells ; Chromosomes ; Cytoplasmic Granules - metabolism ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Genetic Complementation Test ; Metabolism ; Microbiology ; Microscopy, Electron, Transmission ; Miscellaneous ; Molecular biology ; Polyhydroxyalkanoates - metabolism ; Proteins ; Pseudomonas putida ; Pseudomonas putida - genetics ; Pseudomonas putida - metabolism</subject><ispartof>Molecular microbiology, 2011, Vol.79 (2), p.402-418</ispartof><rights>2010 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2010 Blackwell Publishing Ltd.</rights><rights>Copyright Blackwell Publishing Ltd. Jan 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5310-9f978b8e451e2c8de4bd2e917e334f636e3a8b8e1cdfc51a6e4c15bdb775278e3</citedby><cites>FETCH-LOGICAL-c5310-9f978b8e451e2c8de4bd2e917e334f636e3a8b8e1cdfc51a6e4c15bdb775278e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.2010.07450.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.2010.07450.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,4010,27900,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23729346$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21219460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Galán, B</creatorcontrib><creatorcontrib>Dinjaski, N</creatorcontrib><creatorcontrib>Maestro, B</creatorcontrib><creatorcontrib>de Eugenio, L.I</creatorcontrib><creatorcontrib>Escapa, I.F</creatorcontrib><creatorcontrib>Sanz, J.M</creatorcontrib><creatorcontrib>García, J.L</creatorcontrib><creatorcontrib>Prieto, M.A</creatorcontrib><title>Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>The PhaF is a nucleoid-associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; the N-terminal PHA granule-binding domain (phasin domain) and the C-terminal half containing AAKP-like tandem repeats characteristic of the histone H1 family. Although the PhaF binding to PHA granules and its role as transcriptional regulator have been previously demonstrated, the cell physiology meaning of these properties remains unknown. This work demonstrates that PhaF plays a crucial role in granule localization within the cell. TEM and flow cytometry studies of cells producing granules at early growth stage demonstrated that PhaF directs the PHA granules to the centre of the cells, forming a characteristic needle array. Our studies demonstrated the existence of two markedly different cell populations in the strain lacking PhaF protein, i.e. cells with and without PHA. Complementation studies definitively demonstrated a key role of PhaF in granule segregation during the cell division ensuring the equal distribution of granules between daughter cells. In vitro studies showed that PhaF binds DNA through its C-terminal domain in a non-specific manner. All these findings suggested a main role of PhaF in PHA apparatus through interactions with the segregating chromosome.</description><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cells</subject><subject>Chromosomes</subject><subject>Cytoplasmic Granules - metabolism</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Genetic Complementation Test</subject><subject>Metabolism</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Transmission</subject><subject>Miscellaneous</subject><subject>Molecular biology</subject><subject>Polyhydroxyalkanoates - metabolism</subject><subject>Proteins</subject><subject>Pseudomonas putida</subject><subject>Pseudomonas putida - genetics</subject><subject>Pseudomonas putida - metabolism</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkstu1DAUhiMEotPCK4CFhFhl8C2JvWCBKgoVLVSildhZZ2xnxoMnDvaEzjwDL43TDEViA9749v3H55zfRYEInpM8Xq_nhNVVSWUl5hTnU9zwCs93D4rZ_cXDYoZlhUsm6Nej4jilNcaE4Zo9Lo4ooUTyGs-Kn58G7W1wpoSUgnawtQZdreAM9StIrkMmuh82IddtI2jr_eAhIh80bF3oEHQGJbuMdjntQ4v64PervYlhtwf_DbqQQ6JlhG7wd3HQVbKDCZvQQUL9sHUG0Mdryjl9UjxqwSf79DCfFDdn765PP5QXn9-fn769KHXFCC5lKxuxEJZXxFItjOULQ60kjWWMtzWrLYPxnmjT6opAbbkm1cIsmqaijbDspHg1xe1j-D7YtFUbl8baoLNhSEoIgWXdUPlvkuW-M0nrTL74i1yHIXa5DCV4LVnDBM-QmCAdQ0rRtqqPbgNxrwhWo7FqrUb_1OifGo1Vd8aqXZY-O8QfFhtr7oW_nczAywMASYNvc8O1S384NtbDx0TfTNyt83b_3wmoy8vzcZX1zyd9C0HBMuY3br7Q8WcRmd0Rkv0CpVzJvA</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Galán, B</creator><creator>Dinjaski, N</creator><creator>Maestro, B</creator><creator>de Eugenio, L.I</creator><creator>Escapa, I.F</creator><creator>Sanz, J.M</creator><creator>García, J.L</creator><creator>Prieto, M.A</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442</title><author>Galán, B ; Dinjaski, N ; Maestro, B ; de Eugenio, L.I ; Escapa, I.F ; Sanz, J.M ; García, J.L ; Prieto, M.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5310-9f978b8e451e2c8de4bd2e917e334f636e3a8b8e1cdfc51a6e4c15bdb775278e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cells</topic><topic>Chromosomes</topic><topic>Cytoplasmic Granules - metabolism</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Genetic Complementation Test</topic><topic>Metabolism</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Transmission</topic><topic>Miscellaneous</topic><topic>Molecular biology</topic><topic>Polyhydroxyalkanoates - metabolism</topic><topic>Proteins</topic><topic>Pseudomonas putida</topic><topic>Pseudomonas putida - genetics</topic><topic>Pseudomonas putida - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Galán, B</creatorcontrib><creatorcontrib>Dinjaski, N</creatorcontrib><creatorcontrib>Maestro, B</creatorcontrib><creatorcontrib>de Eugenio, L.I</creatorcontrib><creatorcontrib>Escapa, I.F</creatorcontrib><creatorcontrib>Sanz, J.M</creatorcontrib><creatorcontrib>García, J.L</creatorcontrib><creatorcontrib>Prieto, M.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Galán, B</au><au>Dinjaski, N</au><au>Maestro, B</au><au>de Eugenio, L.I</au><au>Escapa, I.F</au><au>Sanz, J.M</au><au>García, J.L</au><au>Prieto, M.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2011</date><risdate>2011</risdate><volume>79</volume><issue>2</issue><spage>402</spage><epage>418</epage><pages>402-418</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>The PhaF is a nucleoid-associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; the N-terminal PHA granule-binding domain (phasin domain) and the C-terminal half containing AAKP-like tandem repeats characteristic of the histone H1 family. Although the PhaF binding to PHA granules and its role as transcriptional regulator have been previously demonstrated, the cell physiology meaning of these properties remains unknown. This work demonstrates that PhaF plays a crucial role in granule localization within the cell. TEM and flow cytometry studies of cells producing granules at early growth stage demonstrated that PhaF directs the PHA granules to the centre of the cells, forming a characteristic needle array. Our studies demonstrated the existence of two markedly different cell populations in the strain lacking PhaF protein, i.e. cells with and without PHA. Complementation studies definitively demonstrated a key role of PhaF in granule segregation during the cell division ensuring the equal distribution of granules between daughter cells. In vitro studies showed that PhaF binds DNA through its C-terminal domain in a non-specific manner. All these findings suggested a main role of PhaF in PHA apparatus through interactions with the segregating chromosome.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21219460</pmid><doi>10.1111/j.1365-2958.2010.07450.x</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0950-382X |
ispartof | Molecular microbiology, 2011, Vol.79 (2), p.402-418 |
issn | 0950-382X 1365-2958 |
language | eng |
recordid | cdi_proquest_miscellaneous_888096729 |
source | Wiley-Blackwell Journals; Wiley-Blackwell Open Access Backfiles; MEDLINE; EZB Electronic Journals Library |
subjects | Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Binding sites Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - metabolism Cells Chromosomes Cytoplasmic Granules - metabolism Flow Cytometry Fundamental and applied biological sciences. Psychology Gene Deletion Genetic Complementation Test Metabolism Microbiology Microscopy, Electron, Transmission Miscellaneous Molecular biology Polyhydroxyalkanoates - metabolism Proteins Pseudomonas putida Pseudomonas putida - genetics Pseudomonas putida - metabolism |
title | Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442 |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T19%3A41%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nucleoid-associated%20PhaF%20phasin%20drives%20intracellular%20location%20and%20segregation%20of%20polyhydroxyalkanoate%20granules%20in%20Pseudomonas%20putida%20KT2442&rft.jtitle=Molecular%20microbiology&rft.au=Gal%C3%A1n,%20B&rft.date=2011&rft.volume=79&rft.issue=2&rft.spage=402&rft.epage=418&rft.pages=402-418&rft.issn=0950-382X&rft.eissn=1365-2958&rft_id=info:doi/10.1111/j.1365-2958.2010.07450.x&rft_dat=%3Cproquest_cross%3E837453926%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=846937384&rft_id=info:pmid/21219460&rfr_iscdi=true |