Liposomes destabilize tRNA during heat stress

Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphoc...

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Veröffentlicht in:	Biotechnology journal 2010-05, Vol.5 (5), p.526-529
Hauptverfasser:	Suga, Keishi, Umakoshi, Hiroshi, Tomita, Hibiki, Tanabe, Tomoyuki, Shimanouchi, Toshinori, Kuboi, Ryoichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemical Engineering C.D Cholesterol - chemistry Cholesterol - metabolism Circular Dichroism Escherichia coli - chemistry Heat stress response Heat-Shock Response Hot Temperature Liposome Liposomes - chemistry Liposomes - metabolism Membrane stress biotechnology Nucleic Acid Conformation Phosphatidylcholines - chemistry Phosphatidylcholines - metabolism RNA, Bacterial - chemistry RNA, Bacterial - metabolism RNA, Transfer - chemistry RNA, Transfer - metabolism tRNA conformation
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Zusammenfassung:	Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (Tm) of tRNA was calculated from normalized θ208. Addition of POPC/Ch liposomes decreased the Tm value of tRNA from 48°C to 38°C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.
ISSN:	1860-6768 1860-7314 1860-7314
DOI:	10.1002/biot.200900289