Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays

In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhe...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (37), p.10329-10331
Hauptverfasser:	Iwasaki, Yasuhiko, Ota, Tatsuya
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotinylation Cell Proliferation Glycols Hexosamines - chemistry Hexosamines - metabolism HL-60 Cells Human Humans Leukemias Polyethylene Glycols - chemistry Sulfhydryl Compounds - chemistry Tissue Array Analysis - methods Viability
Online-Zugang:	Volltext
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