Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays

Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays

In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhe...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (37), p.10329-10331
Hauptverfasser: Iwasaki, Yasuhiko, Ota, Tatsuya
Format: Artikel
Sprache:eng
Schlagworte:
Biotinylation
Cell Proliferation
Glycols
Hexosamines - chemistry
Hexosamines - metabolism
HL-60 Cells
Human
Humans
Leukemias
Polyethylene Glycols - chemistry
Sulfhydryl Compounds - chemistry
Tissue Array Analysis - methods
Viability
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Zusammenfassung:In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.
ISSN:1359-7345
1364-548X
DOI:10.1039/c1cc12948a