Calculation of Singlet Oxygen Dose Using Explicit and Implicit Dose Metrics During Benzoporphyrin Derivative Monoacid Ring A (BPD-MA)-PDT In Vitro and Correlation with MLL Cell Survival
ABSTRACT Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐PDT of MAT‐LyLu cells in vitro. Cells were incubated with BPD‐MA concentrations of 0.1, 0.5 or...
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| Veröffentlicht in: | Photochemistry and photobiology 2011-09, Vol.87 (5), p.1129-1137 |
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| creator | Weston, Mark A. Patterson, Michael S. |
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Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐PDT of MAT‐LyLu cells in vitro. Cells were incubated with BPD‐MA concentrations of 0.1, 0.5 or 2.5 μg mL−1 for 2 h and then treated with 405 nm light under oxygenated and hypoxic conditions. Fluorescence spectra were acquired during treatment, and photobleaching and photoproduct generation were quantified using singular value decomposition of the spectra. Cell survival was measured at set times during the treatment using a colony‐forming assay. The amount of oxygen consumed by PDT per photon absorbed decreased with BPD‐MA intracellular concentration. Survival was correlated with the total amount of oxygen consumed by PDT per unit volume, which is assumed to be equivalent to the amount of singlet oxygen that reacted. A photobleaching‐based singlet oxygen dose metric was also found to predict survival independent of intracellular BPD‐MA concentration. The BPD‐MA photoproduct was bleached during the treatment. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD‐MA concentrations used.
Oxygen consumption, cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐photodynamic therapy (PDT) of MAT‐LyLu cells in vitro. Survival was correlated with the total amount of oxygen consumed by PDT. A photobleaching‐based singlet oxygen dose metric was found to predict survival independent of intracellular BPD‐MA concentration or treatment fluence rate. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD‐MA concentrations used. |
| doi_str_mv | 10.1111/j.1751-1097.2011.00942.x |
| format | Article |
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Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐PDT of MAT‐LyLu cells in vitro. Cells were incubated with BPD‐MA concentrations of 0.1, 0.5 or 2.5 μg mL−1 for 2 h and then treated with 405 nm light under oxygenated and hypoxic conditions. Fluorescence spectra were acquired during treatment, and photobleaching and photoproduct generation were quantified using singular value decomposition of the spectra. Cell survival was measured at set times during the treatment using a colony‐forming assay. The amount of oxygen consumed by PDT per photon absorbed decreased with BPD‐MA intracellular concentration. Survival was correlated with the total amount of oxygen consumed by PDT per unit volume, which is assumed to be equivalent to the amount of singlet oxygen that reacted. A photobleaching‐based singlet oxygen dose metric was also found to predict survival independent of intracellular BPD‐MA concentration. The BPD‐MA photoproduct was bleached during the treatment. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD‐MA concentrations used.
Oxygen consumption, cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐photodynamic therapy (PDT) of MAT‐LyLu cells in vitro. Survival was correlated with the total amount of oxygen consumed by PDT. A photobleaching‐based singlet oxygen dose metric was found to predict survival independent of intracellular BPD‐MA concentration or treatment fluence rate. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD‐MA concentrations used.</description><identifier>ISSN: 0031-8655</identifier><identifier>EISSN: 1751-1097</identifier><identifier>DOI: 10.1111/j.1751-1097.2011.00942.x</identifier><identifier>PMID: 21575000</identifier><identifier>CODEN: PHCBAP</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adenocarcinoma - drug therapy ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Animals ; Cell Line, Tumor ; Cell Survival - drug effects ; Cell Survival - radiation effects ; Cells ; Chemical compounds ; Dose-Response Relationship, Drug ; Fluorescence ; Humans ; Hypoxia ; Kinetics ; Liposomes - chemistry ; Male ; Oxygen ; Oxygen Consumption - drug effects ; Oxygen Consumption - radiation effects ; Photobiology ; Photobleaching ; Photochemotherapy - methods ; Photodynamic therapy ; Photosensitizing Agents - pharmacology ; Porphyrins - pharmacology ; Prostatic Neoplasms - drug therapy ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - pathology ; Radiation Dosage ; Rats ; Singlet Oxygen - analysis ; Singlet Oxygen - metabolism ; Spectrometry, Fluorescence</subject><ispartof>Photochemistry and photobiology, 2011-09, Vol.87 (5), p.1129-1137</ispartof><rights>2011 The Authors. Photochemistry and Photobiology © 2011 The American Society of Photobiology</rights><rights>2011 The Authors. Photochemistry and Photobiology © 2011 The American Society of Photobiology.</rights><rights>Copyright Blackwell Publishing Ltd. Sep/Oct 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4322-ad14e2115f0abada53a12bf8df6e5c0d4d76f66a832484754499b4db511321d73</citedby><cites>FETCH-LOGICAL-c4322-ad14e2115f0abada53a12bf8df6e5c0d4d76f66a832484754499b4db511321d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1751-1097.2011.00942.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1751-1097.2011.00942.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21575000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weston, Mark A.</creatorcontrib><creatorcontrib>Patterson, Michael S.</creatorcontrib><title>Calculation of Singlet Oxygen Dose Using Explicit and Implicit Dose Metrics During Benzoporphyrin Derivative Monoacid Ring A (BPD-MA)-PDT In Vitro and Correlation with MLL Cell Survival</title><title>Photochemistry and photobiology</title><addtitle>Photochem Photobiol</addtitle><description>ABSTRACT
Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐PDT of MAT‐LyLu cells in vitro. Cells were incubated with BPD‐MA concentrations of 0.1, 0.5 or 2.5 μg mL−1 for 2 h and then treated with 405 nm light under oxygenated and hypoxic conditions. Fluorescence spectra were acquired during treatment, and photobleaching and photoproduct generation were quantified using singular value decomposition of the spectra. Cell survival was measured at set times during the treatment using a colony‐forming assay. The amount of oxygen consumed by PDT per photon absorbed decreased with BPD‐MA intracellular concentration. Survival was correlated with the total amount of oxygen consumed by PDT per unit volume, which is assumed to be equivalent to the amount of singlet oxygen that reacted. A photobleaching‐based singlet oxygen dose metric was also found to predict survival independent of intracellular BPD‐MA concentration. The BPD‐MA photoproduct was bleached during the treatment. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD‐MA concentrations used.
Oxygen consumption, cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐photodynamic therapy (PDT) of MAT‐LyLu cells in vitro. Survival was correlated with the total amount of oxygen consumed by PDT. A photobleaching‐based singlet oxygen dose metric was found to predict survival independent of intracellular BPD‐MA concentration or treatment fluence rate. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD‐MA concentrations used.</description><subject>Adenocarcinoma - drug therapy</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Animals</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>Cells</subject><subject>Chemical compounds</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>Kinetics</subject><subject>Liposomes - chemistry</subject><subject>Male</subject><subject>Oxygen</subject><subject>Oxygen Consumption - drug effects</subject><subject>Oxygen Consumption - radiation effects</subject><subject>Photobiology</subject><subject>Photobleaching</subject><subject>Photochemotherapy - methods</subject><subject>Photodynamic therapy</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Porphyrins - pharmacology</subject><subject>Prostatic Neoplasms - drug therapy</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Radiation Dosage</subject><subject>Rats</subject><subject>Singlet Oxygen - analysis</subject><subject>Singlet Oxygen - metabolism</subject><subject>Spectrometry, Fluorescence</subject><issn>0031-8655</issn><issn>1751-1097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1y0zAUhT0MDA2FV2A0bICFjSRL_plhk9qlzUxMDW2BnUa25VapYxnJTh3ejLdDTkIWrNBGutJ3zr2j4zgAQQ_Z9WHloZAiF8E49DBEyIMwJtgbnziz48NTZwahj9wooPTEeWHMCkJE4hA9d04woiGFEM6c3wlvyqHhvVQtUDW4lu1dI3pwNW7vRAtSZQS4NfYSnI9dI0vZA95WYLE-FDsgE72WpQHpoCfyTLS_VKd0d7-1NUiFlhvbYGNB1Speygp8nbg5eHeWp242f-_m6Q1YtOCb7LXaNUiU1uIw1qPs70G2XIJENA24HvTG-jUvnWc1b4x4ddhPndtP5zfJpbu8ulgk86VbEh9jl1eICIwQrSEveMWpzxEu6qiqA0FLWJEqDOog4JGPSURCSkgcF6QqKEI-RlXonzpv976dVj8HYXq2lqa0k_BWqMGwKLJfSWAALfnmH3KlBt3a4ViMoxhRHGALRXuo1MoYLWrWabnmessQZFO4bMWmDNmUIZvCZbtw2Wilrw_-Q7EW1VH4N00LfNwDj7IR2_82Zvllbg9W7u7l0vRiPMq5fmBB6IeUff98wciPL1mWxzHD_h8mJsGk</recordid><startdate>201109</startdate><enddate>201109</enddate><creator>Weston, Mark A.</creator><creator>Patterson, Michael S.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201109</creationdate><title>Calculation of Singlet Oxygen Dose Using Explicit and Implicit Dose Metrics During Benzoporphyrin Derivative Monoacid Ring A (BPD-MA)-PDT In Vitro and Correlation with MLL Cell Survival</title><author>Weston, Mark A. ; Patterson, Michael S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4322-ad14e2115f0abada53a12bf8df6e5c0d4d76f66a832484754499b4db511321d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adenocarcinoma - drug therapy</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Animals</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - radiation effects</topic><topic>Cells</topic><topic>Chemical compounds</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Hypoxia</topic><topic>Kinetics</topic><topic>Liposomes - chemistry</topic><topic>Male</topic><topic>Oxygen</topic><topic>Oxygen Consumption - drug effects</topic><topic>Oxygen Consumption - radiation effects</topic><topic>Photobiology</topic><topic>Photobleaching</topic><topic>Photochemotherapy - methods</topic><topic>Photodynamic therapy</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Porphyrins - pharmacology</topic><topic>Prostatic Neoplasms - drug therapy</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Radiation Dosage</topic><topic>Rats</topic><topic>Singlet Oxygen - analysis</topic><topic>Singlet Oxygen - metabolism</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weston, Mark A.</creatorcontrib><creatorcontrib>Patterson, Michael S.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Photochemistry and photobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weston, Mark A.</au><au>Patterson, Michael S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calculation of Singlet Oxygen Dose Using Explicit and Implicit Dose Metrics During Benzoporphyrin Derivative Monoacid Ring A (BPD-MA)-PDT In Vitro and Correlation with MLL Cell Survival</atitle><jtitle>Photochemistry and photobiology</jtitle><addtitle>Photochem Photobiol</addtitle><date>2011-09</date><risdate>2011</risdate><volume>87</volume><issue>5</issue><spage>1129</spage><epage>1137</epage><pages>1129-1137</pages><issn>0031-8655</issn><eissn>1751-1097</eissn><coden>PHCBAP</coden><abstract>ABSTRACT
Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐PDT of MAT‐LyLu cells in vitro. Cells were incubated with BPD‐MA concentrations of 0.1, 0.5 or 2.5 μg mL−1 for 2 h and then treated with 405 nm light under oxygenated and hypoxic conditions. Fluorescence spectra were acquired during treatment, and photobleaching and photoproduct generation were quantified using singular value decomposition of the spectra. Cell survival was measured at set times during the treatment using a colony‐forming assay. The amount of oxygen consumed by PDT per photon absorbed decreased with BPD‐MA intracellular concentration. Survival was correlated with the total amount of oxygen consumed by PDT per unit volume, which is assumed to be equivalent to the amount of singlet oxygen that reacted. A photobleaching‐based singlet oxygen dose metric was also found to predict survival independent of intracellular BPD‐MA concentration. The BPD‐MA photoproduct was bleached during the treatment. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD‐MA concentrations used.
Oxygen consumption, cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD‐MA)‐photodynamic therapy (PDT) of MAT‐LyLu cells in vitro. Survival was correlated with the total amount of oxygen consumed by PDT. A photobleaching‐based singlet oxygen dose metric was found to predict survival independent of intracellular BPD‐MA concentration or treatment fluence rate. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD‐MA concentrations used.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21575000</pmid><doi>10.1111/j.1751-1097.2011.00942.x</doi><tpages>9</tpages></addata></record> |
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| subjects | Adenocarcinoma - drug therapy Adenocarcinoma - metabolism Adenocarcinoma - pathology Animals Cell Line, Tumor Cell Survival - drug effects Cell Survival - radiation effects Cells Chemical compounds Dose-Response Relationship, Drug Fluorescence Humans Hypoxia Kinetics Liposomes - chemistry Male Oxygen Oxygen Consumption - drug effects Oxygen Consumption - radiation effects Photobiology Photobleaching Photochemotherapy - methods Photodynamic therapy Photosensitizing Agents - pharmacology Porphyrins - pharmacology Prostatic Neoplasms - drug therapy Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Radiation Dosage Rats Singlet Oxygen - analysis Singlet Oxygen - metabolism Spectrometry, Fluorescence |
| title | Calculation of Singlet Oxygen Dose Using Explicit and Implicit Dose Metrics During Benzoporphyrin Derivative Monoacid Ring A (BPD-MA)-PDT In Vitro and Correlation with MLL Cell Survival |
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