Gene Silencing by siRNA Microhydrogels via Polymeric Nanoscale Condensation

The first attempt to prepare biologically active siRNA-based microhydrogels is reported. The self-assembled microhydrogels were fabricated using sense/antisense complementary hybridization between single-stranded linear and Y-shaped trimeric siRNAs. The siRNA microhydrogels were condensed using a po...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of the American Chemical Society 2011-09, Vol.133 (35), p.13914-13917
Hauptverfasser:	Hong, Cheol Am, Lee, Soo Hyeon, Kim, Jee Seon, Park, Ji Won, Bae, Ki Hyun, Mok, Hyejung, Park, Tae Gwan, Lee, Haeshin
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line, Tumor Gene Silencing Humans Hydrogels - chemistry Nanoparticles - chemistry Polymers - chemistry RNA, Small Interfering - administration & dosage RNA, Small Interfering - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The first attempt to prepare biologically active siRNA-based microhydrogels is reported. The self-assembled microhydrogels were fabricated using sense/antisense complementary hybridization between single-stranded linear and Y-shaped trimeric siRNAs. The siRNA microhydrogels were condensed using a popular cationic polymer such as LPEI to form compact, stable siRNA/polymeric nanoparticles that exhibited superb cellular uptake efficiency and gene silencing activity.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja2056984