Immunisation with the glycolytic enzyme enolase confers effective protection against Candida albicans infection in mice

Highlights ► Mice were immunized with recombinant enolase. ► High levels of IgG1 and IgG2a were induced in immune serum. Killing capacity of neutrophils enhanced after yeasts were opsonized with anti-serum. Fungal burdens reduced after antibody were passive transferred. ► Both Th1 cytokine IL-12 and...

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Veröffentlicht in:	Vaccine 2011-07, Vol.29 (33), p.5526-5533
Hauptverfasser:	Li, Wen qing, Hu, Xu chu, Zhang, Xiaohuan, Ge, Yanping, Zhao, Sainan, Hu, Yan, Ashman, Robert B
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity chromatography Allergy and Immunology Animal Structures - microbiology Animal Structures - pathology Animals antibodies Antibodies, Fungal - blood Antibody Antigens, Fungal - administration & dosage Antigens, Fungal - immunology antiserum Applied microbiology Biological and medical sciences blood serum brain Candida albicans Candida albicans - enzymology Candida albicans - immunology Candidiasis Candidiasis - immunology Candidiasis - microbiology Candidiasis - pathology Candidiasis - prevention & control cell walls Cloning Colleges & universities Colony Count, Microbial Cytokine Cytokines Cytokines - blood Disease Models, Animal Enolase Enzymes Female Fundamental and applied biological sciences. Psychology Fungal Vaccines - administration & dosage Fungal Vaccines - immunology fungi glycolysis Histocytochemistry histopathology humans Immunization Immunization, Secondary - methods immunocompromised population immunoglobulin G Immunoglobulin G - blood Infections Injections, Subcutaneous interleukin-10 interleukin-12 interleukin-8 kidneys liver Membranes Mice Mice, Inbred C57BL Microbiology Miscellaneous molecular cloning Mortality Mycology neutrophils Opsonin Proteins - blood pathogens Phosphopyruvate Hydratase - administration & dosage Phosphopyruvate Hydratase - immunology protective effect Proteins Rodent Diseases - immunology Rodent Diseases - prevention & control spleen vaccination Vaccination - methods Vaccine vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - immunology Western blotting Yeasts
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Zusammenfassung:	Highlights ► Mice were immunized with recombinant enolase. ► High levels of IgG1 and IgG2a were induced in immune serum. Killing capacity of neutrophils enhanced after yeasts were opsonized with anti-serum. Fungal burdens reduced after antibody were passive transferred. ► Both Th1 cytokine IL-12 and Th2 cytokine IL-10 increased in immune serum. ► Enolase can effectively protect against systemic infection caused by two strains of Candida albicans differing in virulence in mice.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2011.05.030