Proteome profile of the developing maize (Zea mays L.) rachis

In this study, we performed the first high‐throughput proteomic analysis of developing rachis (cob) from maize genotype Mp313E. Using two proteomic approaches, 2‐DE and 2‐D LC, we identified 967 proteins. A 2‐D proteome reference map was established. Functional classification of identified proteins...

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Veröffentlicht in:	Proteomics (Weinheim) 2010-08, Vol.10 (16), p.3051-3055
Hauptverfasser:	Pechanova, Olga, Pechan, Tibor, Ozkan, Seval, McCarthy, Fiona M., Williams, W. Paul, Luthe, Dawn S.
Format:	Artikel
Sprache:	eng
Schlagworte:	2-D LC-MS/MS 2-DE Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, Liquid Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology Miscellaneous Peptide Fragments - chemistry Peptide Fragments - metabolism Plant Proteins - chemistry Plant Proteins - metabolism Plant proteomics Proteins Proteome - chemistry Proteome - metabolism Rachis Tandem Mass Spectrometry Trypsin - metabolism Zea mays Zea mays - chemistry
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Zusammenfassung:	In this study, we performed the first high‐throughput proteomic analysis of developing rachis (cob) from maize genotype Mp313E. Using two proteomic approaches, 2‐DE and 2‐D LC, we identified 967 proteins. A 2‐D proteome reference map was established. Functional classification of identified proteins revealed that proteins involved in various cellular metabolisms, response to stimulus and transport, were the most abundant.
ISSN:	1615-9853 1615-9861 1615-9861
DOI:	10.1002/pmic.200900833