Reduction in miR-141 is Induced by Leukemia Inhibitory Factor and Inhibits Proliferation in Choriocarcinoma Cell Line JEG-3

Citation Morales‐Prieto DM, Schleussner E, Markert UR. Reduction in miR‐141 is Induced by Leukemia Inhibitory Factor and Inhibits Proliferation in Choriocarcinoma Cell line JEG‐3. Am J Reprod Immunol 2011; 66 (Suppl. 1): 57–62 Problem Starting from the peri‐implantation period, leukemia inhibitory factor (LIF) is a major regulator of trophoblast functions. Micro‐RNAs (miRNA) are short non‐coding RNA sequences, which regulate expression of genes at post‐transcriptional level. The influence of LIF on miRNA expression in trophoblastic cells has not yet been analyzed and was focus of this investigation. Method of study JEG‐3 choriocarcinoma cells have been stimulated with LIF for 1, 2, 4, 6, and 24 hr. The expression of miR‐9, miR‐141, miR‐21, miR‐93, and let‐7g has been analyzed by real‐time PCR. Subsequently, miR‐141 has been silenced and over‐expressed to test its role in the proliferation of JEG‐3 cells after 24 and 48 hr. Results MiR‐141 has been significantly downregulated by more than 50% after LIF stimulation, while miR‐21 and miR‐93 expression has been significantly upregulated. Silencing of miR‐141 completely inhibited the proliferation of JEG‐3 cells, while over‐expression had no effect. Conclusion LIF regulates expression of miRNA in trophoblastic cells, which may be responsible for several functional effects induced by LIF.