Carboxymethyl-chitin promotes chondrogenesis by inducing the production of growth factors from immune cells
Many techniques have been tested for their ability to restore cartilage defects, but several problems still remain in the complete healing of injured cartilage. In our previous study, we found that a carboxymethyl‐chitin/β‐tricalcium phosphate (CM‐chitin/β‐TCP) composite induced cartilage regenerati...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2010-09, Vol.94A (4), p.1034-1041 |
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| container_title | Journal of biomedical materials research. Part A |
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| creator | Kariya, Hiroyuki Yoshihara, Yusuke Nakao, Yumiko Sakurai, Nobuko Ueno, Masaru Hashimoto, Masahito Suda, Yasuo |
| description | Many techniques have been tested for their ability to restore cartilage defects, but several problems still remain in the complete healing of injured cartilage. In our previous study, we found that a carboxymethyl‐chitin/β‐tricalcium phosphate (CM‐chitin/β‐TCP) composite induced cartilage regeneration in the osteochondral defects of rabbits in vivo. We also found that CM‐chitin stimulated peritoneal exudate cells (PEC) in mice and induced several kinds of inflammatory cytokines and transforming growth factor beta‐1 (TGF‐β1). In this study, we examined whether CM‐chitin is responsible for the induction of chondrogenesis via the production of TGF‐β1 in vitro. The murine pluripotent cell line C3H10T1/2 was maintained as a micromass culture in conditioned medium prepared from PEC stimulated with and without CM‐chitin. CM‐chitin–conditioned medium induced RNA expression of the chondrogenic‐factor Sox9 and the matrix proteins aggrecan, Col2a1, and Comp. Their expression levels were decreased in the presence of anti‐TGF‐β1 antibody. The micromass tissues cultured in CM‐chitin conditioned medium at day 21 were clearly stained by Toluidine blue or Alcian blue (histological staining) and collagen II antibody (immunohistological staining), showing the expression of acidic glycosaminoglycan and type II collagen. Similar results were observed in micromass tissue stimulated with TGF‐β1 as a positive control. However, no chondrogenesis occurred when CM‐chitin was added directly to a C3H10T1/2 cell culture. These results indicated that CM‐chitin is a potent inducer of chondrogenesis via the induction of TGF‐β1 in immune cells. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010. |
| doi_str_mv | 10.1002/jbm.a.32771 |
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In our previous study, we found that a carboxymethyl‐chitin/β‐tricalcium phosphate (CM‐chitin/β‐TCP) composite induced cartilage regeneration in the osteochondral defects of rabbits in vivo. We also found that CM‐chitin stimulated peritoneal exudate cells (PEC) in mice and induced several kinds of inflammatory cytokines and transforming growth factor beta‐1 (TGF‐β1). In this study, we examined whether CM‐chitin is responsible for the induction of chondrogenesis via the production of TGF‐β1 in vitro. The murine pluripotent cell line C3H10T1/2 was maintained as a micromass culture in conditioned medium prepared from PEC stimulated with and without CM‐chitin. CM‐chitin–conditioned medium induced RNA expression of the chondrogenic‐factor Sox9 and the matrix proteins aggrecan, Col2a1, and Comp. Their expression levels were decreased in the presence of anti‐TGF‐β1 antibody. The micromass tissues cultured in CM‐chitin conditioned medium at day 21 were clearly stained by Toluidine blue or Alcian blue (histological staining) and collagen II antibody (immunohistological staining), showing the expression of acidic glycosaminoglycan and type II collagen. Similar results were observed in micromass tissue stimulated with TGF‐β1 as a positive control. However, no chondrogenesis occurred when CM‐chitin was added directly to a C3H10T1/2 cell culture. These results indicated that CM‐chitin is a potent inducer of chondrogenesis via the induction of TGF‐β1 in immune cells. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.</description><identifier>ISSN: 1549-3296</identifier><identifier>ISSN: 1552-4965</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.32771</identifier><identifier>PMID: 20694970</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Antibodies ; Antibodies - immunology ; Applied sciences ; Biological and medical sciences ; Biomarkers - metabolism ; Biomedical materials ; Cartilage ; Cell Death - drug effects ; Cell Line ; Cell physiology ; Cell Proliferation - drug effects ; Chitin - analogs & derivatives ; Chitin - pharmacology ; chondrogenesis ; Chondrogenesis - drug effects ; Chondrogenesis - genetics ; CM-chitin ; Conditioning ; Culture ; Defects ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Immune System - cytology ; Intercellular Signaling Peptides and Proteins - biosynthesis ; Medical sciences ; Mice ; micromass culture ; Mineralization, calcification ; Molecular and cellular biology ; Natural polymers ; PEC ; Physicochemistry of polymers ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - drug effects ; Pluripotent Stem Cells - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Staining ; Starch and polysaccharides ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical implants ; Technology. Biomaterials. Equipments ; TGF-β ; Transforming Growth Factor beta1 - immunology ; Transforming Growth Factor beta1 - pharmacology</subject><ispartof>Journal of biomedical materials research. Part A, 2010-09, Vol.94A (4), p.1034-1041</ispartof><rights>Copyright © 2010 Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>(c) 2010 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4951-c14416f4c3f2396804b26eb07fae9f1f56751a78dfdbf10ca991251ecf26b89a3</citedby><cites>FETCH-LOGICAL-c4951-c14416f4c3f2396804b26eb07fae9f1f56751a78dfdbf10ca991251ecf26b89a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.32771$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.32771$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23164209$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20694970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kariya, Hiroyuki</creatorcontrib><creatorcontrib>Yoshihara, Yusuke</creatorcontrib><creatorcontrib>Nakao, Yumiko</creatorcontrib><creatorcontrib>Sakurai, Nobuko</creatorcontrib><creatorcontrib>Ueno, Masaru</creatorcontrib><creatorcontrib>Hashimoto, Masahito</creatorcontrib><creatorcontrib>Suda, Yasuo</creatorcontrib><title>Carboxymethyl-chitin promotes chondrogenesis by inducing the production of growth factors from immune cells</title><title>Journal of biomedical materials research. Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Many techniques have been tested for their ability to restore cartilage defects, but several problems still remain in the complete healing of injured cartilage. In our previous study, we found that a carboxymethyl‐chitin/β‐tricalcium phosphate (CM‐chitin/β‐TCP) composite induced cartilage regeneration in the osteochondral defects of rabbits in vivo. We also found that CM‐chitin stimulated peritoneal exudate cells (PEC) in mice and induced several kinds of inflammatory cytokines and transforming growth factor beta‐1 (TGF‐β1). In this study, we examined whether CM‐chitin is responsible for the induction of chondrogenesis via the production of TGF‐β1 in vitro. The murine pluripotent cell line C3H10T1/2 was maintained as a micromass culture in conditioned medium prepared from PEC stimulated with and without CM‐chitin. CM‐chitin–conditioned medium induced RNA expression of the chondrogenic‐factor Sox9 and the matrix proteins aggrecan, Col2a1, and Comp. Their expression levels were decreased in the presence of anti‐TGF‐β1 antibody. The micromass tissues cultured in CM‐chitin conditioned medium at day 21 were clearly stained by Toluidine blue or Alcian blue (histological staining) and collagen II antibody (immunohistological staining), showing the expression of acidic glycosaminoglycan and type II collagen. Similar results were observed in micromass tissue stimulated with TGF‐β1 as a positive control. However, no chondrogenesis occurred when CM‐chitin was added directly to a C3H10T1/2 cell culture. These results indicated that CM‐chitin is a potent inducer of chondrogenesis via the induction of TGF‐β1 in immune cells. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies - immunology</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Biomedical materials</subject><subject>Cartilage</subject><subject>Cell Death - drug effects</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Cell Proliferation - drug effects</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - pharmacology</subject><subject>chondrogenesis</subject><subject>Chondrogenesis - drug effects</subject><subject>Chondrogenesis - genetics</subject><subject>CM-chitin</subject><subject>Conditioning</subject><subject>Culture</subject><subject>Defects</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Immune System - cytology</subject><subject>Intercellular Signaling Peptides and Proteins - biosynthesis</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>micromass culture</subject><subject>Mineralization, calcification</subject><subject>Molecular and cellular biology</subject><subject>Natural polymers</subject><subject>PEC</subject><subject>Physicochemistry of polymers</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - drug effects</subject><subject>Pluripotent Stem Cells - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Staining</subject><subject>Starch and polysaccharides</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgical implants</subject><subject>Technology. Biomaterials. Equipments</subject><subject>TGF-β</subject><subject>Transforming Growth Factor beta1 - immunology</subject><subject>Transforming Growth Factor beta1 - pharmacology</subject><issn>1549-3296</issn><issn>1552-4965</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0clv1DAUB-AIgegCJ-7IFwQSyuDnNT6WmS5AC5eiHi3HY8-4TeJiJ2rnvydhpuXWnvwsfW-RfkXxDvAMMCZfrut2ZmaUSAkvin3gnJRMCf5yqpkqKVFirzjI-XrEAnPyutgjWCimJN4vbuYm1fF-07p-vWlKuw596NBtim3sXUZ2HbtliivXuRwyqjcodMvBhm6F-rWb3PjrQ-xQ9GiV4l2_Rt7YPqaM_DgEhbYdOoesa5r8pnjlTZPd2917WPw-Ob6cn5Xnv06_zY_OS8sUh9ICYyA8s9QTqkSFWU2Eq7H0xikPngvJwchq6Ze1B2yNUkA4OOuJqCtl6GHxcTt3PO_P4HKv25CnC0zn4pB1VVFMGAB_XgrFK4Y5fVZKVinOgEzy05MShARKQVI10s9balPMOTmvb1NoTdpowHrKVo_ZaqP_ZTvq97vBQ9265aN9CHMEH3bAZGsan0xnQ_7vKAhG8LQWtu4uNG7z1E79_evFw_Jy2xNy7-4fe0y60UJSyfXVz1P94_Jksbi4Wugz-hcmrcwm</recordid><startdate>20100915</startdate><enddate>20100915</enddate><creator>Kariya, Hiroyuki</creator><creator>Yoshihara, Yusuke</creator><creator>Nakao, Yumiko</creator><creator>Sakurai, Nobuko</creator><creator>Ueno, Masaru</creator><creator>Hashimoto, Masahito</creator><creator>Suda, Yasuo</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QO</scope><scope>7T5</scope><scope>H94</scope><scope>P64</scope><scope>7QP</scope></search><sort><creationdate>20100915</creationdate><title>Carboxymethyl-chitin promotes chondrogenesis by inducing the production of growth factors from immune cells</title><author>Kariya, Hiroyuki ; Yoshihara, Yusuke ; Nakao, Yumiko ; Sakurai, Nobuko ; Ueno, Masaru ; Hashimoto, Masahito ; Suda, Yasuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4951-c14416f4c3f2396804b26eb07fae9f1f56751a78dfdbf10ca991251ecf26b89a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies - immunology</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Biomedical materials</topic><topic>Cartilage</topic><topic>Cell Death - drug effects</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Cell Proliferation - drug effects</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - pharmacology</topic><topic>chondrogenesis</topic><topic>Chondrogenesis - drug effects</topic><topic>Chondrogenesis - genetics</topic><topic>CM-chitin</topic><topic>Conditioning</topic><topic>Culture</topic><topic>Defects</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Immune System - cytology</topic><topic>Intercellular Signaling Peptides and Proteins - biosynthesis</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>micromass culture</topic><topic>Mineralization, calcification</topic><topic>Molecular and cellular biology</topic><topic>Natural polymers</topic><topic>PEC</topic><topic>Physicochemistry of polymers</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - drug effects</topic><topic>Pluripotent Stem Cells - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Staining</topic><topic>Starch and polysaccharides</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgical implants</topic><topic>Technology. Biomaterials. Equipments</topic><topic>TGF-β</topic><topic>Transforming Growth Factor beta1 - immunology</topic><topic>Transforming Growth Factor beta1 - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kariya, Hiroyuki</creatorcontrib><creatorcontrib>Yoshihara, Yusuke</creatorcontrib><creatorcontrib>Nakao, Yumiko</creatorcontrib><creatorcontrib>Sakurai, Nobuko</creatorcontrib><creatorcontrib>Ueno, Masaru</creatorcontrib><creatorcontrib>Hashimoto, Masahito</creatorcontrib><creatorcontrib>Suda, Yasuo</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kariya, Hiroyuki</au><au>Yoshihara, Yusuke</au><au>Nakao, Yumiko</au><au>Sakurai, Nobuko</au><au>Ueno, Masaru</au><au>Hashimoto, Masahito</au><au>Suda, Yasuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carboxymethyl-chitin promotes chondrogenesis by inducing the production of growth factors from immune cells</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2010-09-15</date><risdate>2010</risdate><volume>94A</volume><issue>4</issue><spage>1034</spage><epage>1041</epage><pages>1034-1041</pages><issn>1549-3296</issn><issn>1552-4965</issn><eissn>1552-4965</eissn><abstract>Many techniques have been tested for their ability to restore cartilage defects, but several problems still remain in the complete healing of injured cartilage. In our previous study, we found that a carboxymethyl‐chitin/β‐tricalcium phosphate (CM‐chitin/β‐TCP) composite induced cartilage regeneration in the osteochondral defects of rabbits in vivo. We also found that CM‐chitin stimulated peritoneal exudate cells (PEC) in mice and induced several kinds of inflammatory cytokines and transforming growth factor beta‐1 (TGF‐β1). In this study, we examined whether CM‐chitin is responsible for the induction of chondrogenesis via the production of TGF‐β1 in vitro. The murine pluripotent cell line C3H10T1/2 was maintained as a micromass culture in conditioned medium prepared from PEC stimulated with and without CM‐chitin. CM‐chitin–conditioned medium induced RNA expression of the chondrogenic‐factor Sox9 and the matrix proteins aggrecan, Col2a1, and Comp. Their expression levels were decreased in the presence of anti‐TGF‐β1 antibody. The micromass tissues cultured in CM‐chitin conditioned medium at day 21 were clearly stained by Toluidine blue or Alcian blue (histological staining) and collagen II antibody (immunohistological staining), showing the expression of acidic glycosaminoglycan and type II collagen. Similar results were observed in micromass tissue stimulated with TGF‐β1 as a positive control. However, no chondrogenesis occurred when CM‐chitin was added directly to a C3H10T1/2 cell culture. These results indicated that CM‐chitin is a potent inducer of chondrogenesis via the induction of TGF‐β1 in immune cells. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20694970</pmid><doi>10.1002/jbm.a.32771</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antibodies Antibodies - immunology Applied sciences Biological and medical sciences Biomarkers - metabolism Biomedical materials Cartilage Cell Death - drug effects Cell Line Cell physiology Cell Proliferation - drug effects Chitin - analogs & derivatives Chitin - pharmacology chondrogenesis Chondrogenesis - drug effects Chondrogenesis - genetics CM-chitin Conditioning Culture Defects Exact sciences and technology Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Immune System - cytology Intercellular Signaling Peptides and Proteins - biosynthesis Medical sciences Mice micromass culture Mineralization, calcification Molecular and cellular biology Natural polymers PEC Physicochemistry of polymers Pluripotent Stem Cells - cytology Pluripotent Stem Cells - drug effects Pluripotent Stem Cells - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Staining Starch and polysaccharides Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgical implants Technology. Biomaterials. Equipments TGF-β Transforming Growth Factor beta1 - immunology Transforming Growth Factor beta1 - pharmacology |
| title | Carboxymethyl-chitin promotes chondrogenesis by inducing the production of growth factors from immune cells |
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