Ethylene removal evaluation and bacterial community analysis of vermicompost as biofilter material

► The vermicompost with indigenous microorganisms as bed material is evaluated for ethylene removal through batch test and biofilter experiment. ► Temporal and spatial dynamics of bacterial community in the vermicompost-biofilter under different ethylene loads are also characterized. ► The vermicompost is a promising bed material for ethylene degradation. ► Pseudomonads and Actinobacteria are predominant during ethylene biofiltration in the vermicompost-biofilter. Biofiltration of ethylene provides an environmentally friendly and economically beneficial option relative to physical/chemical removal, where selection of appropriate bed material is crucial. Here the vermicompost with indigenous microorganisms as bed material was evaluated for ethylene removal through batch test and biofilter experiment. Temporal and spatial dynamics of bacterial community in the vermicompost-biofilter under different ethylene loads were characterized by culture and denaturing gradient gel electrophoresis (DGGE) methods. The results showed that ethylene was effectively degraded by the vermicompost under conditions of 25–50% moisture content and 25–35 °C temperature. The vermicompost-biofilter achieved nearly 100% ethylene removal up to an inlet load of 11 mg m −3 h −1. Local nitrogen lack of the vermicompost in the biofilter was observed over operation time, but the change of pH was slight. DGGE analysis demonstrated that the bacterial abundance and community structure of vermicompost-biofilter varied with the height of biofilter under different ethylene loads. Pseudomonads and Actinobacteria were predominant in the biofilter throughout the whole experiment.