Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization

PURPOSE OF WORK: To clone, express and characterize uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD) from Lactobacillus reuteri. Some strains of Lb. reuteri produce cobalamin (vitamin B12). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex me...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology letters 2011-08, Vol.33 (8), p.1625-1632
Hauptverfasser:	Vannini, Verónica, Rodríguez, Adriana, Vera, José L, de Valdéz, Graciela F, Taranto, María P, Sesma, Fernando
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Applied Microbiology Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - chemistry Bacterial Proteins - genetics Base Sequence biochemical pathways Biochemistry Biological and medical sciences Biomedical and Life Sciences Biosynthesis Biotechnology Cloning Cloning, Molecular Computer Simulation enzymes Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Gene expression genes Lactobacillus reuteri Lactobacillus reuteri - enzymology Lactobacillus reuteri - genetics Life Sciences Methyltransferases - biosynthesis Methyltransferases - chemistry Methyltransferases - genetics Microbiology Molecular Sequence Data Original Research Paper Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Spectrometry, Fluorescence Uroporphyrinogen III Synthetase - biosynthesis Uroporphyrinogen III Synthetase - chemistry Uroporphyrinogen III Synthetase - genetics vitamin B12
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PURPOSE OF WORK: To clone, express and characterize uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD) from Lactobacillus reuteri. Some strains of Lb. reuteri produce cobalamin (vitamin B12). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy L-methionine uroprophyrinogen III methyltransferase/synthase (CobA/HemD), a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/s10529-011-0609-5