Targeted pH-dependent fluorescent activity-based cathepsin probes

Bifunctional, pH-activatable BODIPY dyes were developed and incorporated in mannose cluster-containing activity-based probes for cysteine proteases. Mannose receptor-dependent uptake of the probes in dendritic cells, followed by trafficking to acidic cellular compartments resulted in fluorescence as...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2011-09, Vol.47 (33), p.9363-9365
Hauptverfasser:	Hoogendoorn, Sascha, Habets, Kim L, Passemard, Solène, Kuiper, Johan, van der Marel, Gijsbert A, Florea, Bogdan I, Overkleeft, Herman S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Boron Compounds - chemistry Cathepsins - metabolism Cellular Clusters Compartments Cysteine Dendritic Cells - metabolism Dyes Fluorescence Fluorescent Dyes - chemistry Hydrogen-Ion Concentration Leucine - analogs & derivatives Leucine - chemistry Mannose Mannose - chemistry Mice Microscopy, Fluorescence Uptakes
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Beschreibung
Zusammenfassung:	Bifunctional, pH-activatable BODIPY dyes were developed and incorporated in mannose cluster-containing activity-based probes for cysteine proteases. Mannose receptor-dependent uptake of the probes in dendritic cells, followed by trafficking to acidic cellular compartments resulted in fluorescence as seen by live-cell imaging, and subsequent cathepsin inhibition.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c1cc12947c