CXCR4 positive cells from Lewis lung carcinoma cell line have cancer metastatic stem cell characteristics

There is increasing evidence that cancer stem cells contribute to the initiation and propagation of many tumor. Therefore, to find out and identify the metastatic tumor stem-like cells in Lewis lung cancer cell line (LLC), the expression of CXCR4 was measured in LLC by flow cytometry and observed by...

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Veröffentlicht in:	Molecular and cellular biochemistry 2011-09, Vol.355 (1-2), p.241-248
Hauptverfasser:	Nian, Wei-Qi, Chen, Fang-Lin, Ao, Xu-Jun, Chen, Zheng-Tang
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, CD - metabolism Antineoplastic Agents - pharmacology ATP Binding Cassette Transporter, Sub-Family G, Member 2 ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Biochemistry Biomedical and Life Sciences Cancer Carcinoma Carcinoma, Lewis Lung - metabolism Carcinoma, Lewis Lung - pathology Carcinoma, Lewis Lung - secondary Cardiology Cell Aggregation Cell Line, Tumor Cell Migration Assays Cell Proliferation - drug effects Cisplatin - pharmacology Drug Resistance, Neoplasm Female Gene Knockout Techniques Life Sciences Lung cancer Lung cancer, Non-small cell Lung Neoplasms - metabolism Lung Neoplasms - pathology Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - metabolism Medical Biochemistry Metastasis Mice Mice, Inbred C57BL Neoplasm Invasiveness Neoplasm Transplantation Neoplastic Stem Cells - drug effects Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Oncology Receptor, IGF Type 1 - genetics Receptor, IGF Type 1 - metabolism Receptors, CXCR4 - genetics Receptors, CXCR4 - metabolism RNA Stem cells Toy industry Tumors Vascular endothelial growth factor Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism
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Zusammenfassung:	There is increasing evidence that cancer stem cells contribute to the initiation and propagation of many tumor. Therefore, to find out and identify the metastatic tumor stem-like cells in Lewis lung cancer cell line (LLC), the expression of CXCR4 was measured in LLC by flow cytometry and observed by laser scanning confocal microscope (LSCM). After the CXCR4 + LLC cell was isolated from LLC by magnetic cell sorting, its properties were evaluated by their tumorigenic and metastatic potentials. CXCR4 + cells were counted for 0.18% of the total number of LLC, and immunofluorescent staining cells were identified by LSCM. CXCR4 + LLC suspension cultured in a serum-free medium, cell spheres expressed a high level of Sca-1. The chemotherapy sensitivity to cisplatin of CXCR4 + LLC was lower than that of CXCR4 − LLC. The expression of ABCG2 and IGF1R mRNA in CXCR4 + LLC was higher than that in CXCR4 − LLC ( P
ISSN:	0300-8177 1573-4919
DOI:	10.1007/s11010-011-0860-z