Effects of an aqueous extract of Croton bonplandianum Baill in rats

Abstract We have investigated the cytotoxic and biochemical effects of injecting aqueous phytoextract of Croton bonplandianum (Baill) leaves in male rats. Subchronic dosages of 3.25, 4.65 and 6.97 mg phytoextract g−1 b. wt. week−1 were administered to rats. To test ameliorative effects, rats were injected with phytoextract mixed with 0.2 mg g−1 b. wt. of cyclophosphamide. Positive controls received only cyclophosphamide, while negative control groups were kept on normal diet and water. Our results demonstrate that phytoextract did not induce micronuclei formation in rats and shows insignificant amelioration ( P < 0.05). However, differences in serum LDH isoenzymes, ALP, SGOT, SGPT activities and bilirubin were remarkable and displayed dose as well as duration dependent variations. The most outstanding observation of this study was the release of cardiac TnI in sera of rats injected with 6.97 mg g−1 b. wt. of phytoextract for 21 days. Our findings suggest that at the highest concentrations used here phytoextract of C. bonplandianum is not clastogenic; instead it is cardio- and hepatotoxic.