Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment

Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment

The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in...

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Veröffentlicht in:Review of scientific instruments 2011-05, Vol.82 (5), p.054302-054302-8
Hauptverfasser: Khuc, Mai-Thu, Mendonça, Lucille, Sharma, Sapna, Solinas, Xavier, Volk, Martin, Hache, François
Format: Artikel
Sprache:eng
Schlagworte:
Absorption
Circular Dichroism - instrumentation
Circular Dichroism - methods
Lasers
Optics
Physics
Polyglutamic Acid - chemistry
Protein Denaturation
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
Temperature
Time Factors
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Zusammenfassung:The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.3592331