Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vectors

The significant reduction in reaction time with Eclipta leaf is an important resultant and was enable nanoparticle biosynthesis methods to compete with other plant assisted biosynthesis. The formed silver nanoparticles were highly stable and showed significant mosquito larvicidal activity. The surface reactivity facilitated by capping enables these functionalized nanoparticles as promising candidates for various pharmaceutical, biomedical and environmental applications. [Display omitted] ► Larvicidal activity of synthesized silver nanoparticles utilizing aqueous extract from Eclipta prostrata was investigated against Culex quinquefasciatus and Anopheles subpictus. ► The synthesized AgNPs characterized by UV–vis spectrum, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared and X-ray diffraction. ► The maximum efficacy was observed in synthesized AgNPs against C. quinquefasciatus (LC50 = 4.56 mg/L; LC90 = 13.14 mg/L). ► The maximum efficacy was observed in synthesized AgNPs against A. subpictus (LC50 = 5.14 mg/L; LC90 = 25.68 mg/L). ► SEM analyses of the synthesized AgNPs were measured 35–60nm in size. ► FTIR identify the biomolecules responsible for the reduction of Ag+ ions. Mosquitoes transmit serious human diseases, causing millions of deaths every year. Use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of synthesized natural products for vector control have been a priority in this area. In this study, larvicidal activity of synthesized silver nanoparticles (AgNPs) utilizing aqueous extract from Eclipta prostrata, a member of the Asteraceae was investigated against fourth instar larvae of filariasis vector, Culex quinquefasciatus say and malaria vector, Anopheles subpictus Grassi (Diptera: Culicidae). The synthesized AgNPs characterized by UV–vis spectrum, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and X-ray diffraction (XRD). SEM analyses of the synthesized AgNPs were clearly distinguishable measured 35–60nm in size. Larvae were exposed to varying concentrations of aqueous extract of synthesized AgNPs for 24h. The maximum efficacy was observed in crude aqueous, and synthesized AgNPs against C. quinquefasciatus (LC50=27.49 and 4.56mg/L; LC90=70.38 and 13.14mg/L), and against A. subpictus (LC50=27.85 and 5.14mg/L; LC90=71.4