Bacillus thuringiensis cry1Ab gene confers resistance to potato againstHelicoverpa armigera (Hubner)
Helicoverpa armigera is one of the important insect pests adversely affecting the yield of potatoes in India. A synthetic gene encoding the insecticidal crystal protein (Cry1Ab) ofBacillus thuringiensis (Bt) has been introduced into five genotypes of potato usingAgrobacterium tumefaciens. Southern a...
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| Veröffentlicht in: | Potato research 2000-06, Vol.43 (2), p.143-152 |
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| container_title | Potato research |
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| creator | Chakrabarti, S. K. Mandaokar, A. D. Shukla, A. Pattanayak, D. Naik, P. S. Sharma, R. P. Kumar, P. A. |
| description | Helicoverpa armigera is one of the important insect pests adversely affecting the yield of potatoes in India. A synthetic gene encoding the insecticidal crystal protein (Cry1Ab) ofBacillus thuringiensis (Bt) has been introduced into five genotypes of potato usingAgrobacterium tumefaciens. Southern analysis of DNA from transgenic plants confirmed the integration and copy number of the transgene. Double-antibody quantitative sandwich ELISA analysis demonstrated high levels of Cry1Ab protein expression in transgenic plants. Insect bioassays on the leaves of transgenic plants showed considerable protection against the larvae ofH. armigera in terms of leaf area consumed and larval weight reduction.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/BF02357955 |
| format | Article |
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| subjects | Bacillus thuringiensis Bioassays Genotypes Helicoverpa armigera Insects Larvae Leaves Potatoes Solanum tuberosum Transgenic plants |
| title | Bacillus thuringiensis cry1Ab gene confers resistance to potato againstHelicoverpa armigera (Hubner) |
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