Bacillus thuringiensis cry1Ab gene confers resistance to potato againstHelicoverpa armigera (Hubner)

Bacillus thuringiensis cry1Ab gene confers resistance to potato againstHelicoverpa armigera (Hubner)

Helicoverpa armigera is one of the important insect pests adversely affecting the yield of potatoes in India. A synthetic gene encoding the insecticidal crystal protein (Cry1Ab) ofBacillus thuringiensis (Bt) has been introduced into five genotypes of potato usingAgrobacterium tumefaciens. Southern a...

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Veröffentlicht in:Potato research 2000-06, Vol.43 (2), p.143-152
Hauptverfasser: Chakrabarti, S. K., Mandaokar, A. D., Shukla, A., Pattanayak, D., Naik, P. S., Sharma, R. P., Kumar, P. A.
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus thuringiensis
Bioassays
Genotypes
Helicoverpa armigera
Insects
Larvae
Leaves
Potatoes
Solanum tuberosum
Transgenic plants
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Zusammenfassung:Helicoverpa armigera is one of the important insect pests adversely affecting the yield of potatoes in India. A synthetic gene encoding the insecticidal crystal protein (Cry1Ab) ofBacillus thuringiensis (Bt) has been introduced into five genotypes of potato usingAgrobacterium tumefaciens. Southern analysis of DNA from transgenic plants confirmed the integration and copy number of the transgene. Double-antibody quantitative sandwich ELISA analysis demonstrated high levels of Cry1Ab protein expression in transgenic plants. Insect bioassays on the leaves of transgenic plants showed considerable protection against the larvae ofH. armigera in terms of leaf area consumed and larval weight reduction.[PUBLICATION ABSTRACT]
ISSN:0014-3065
1871-4528
DOI:10.1007/BF02357955